GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥90%(SDS-PAGE)
Expression System
HEK293
Accession #
P07237
Protein Tag
C-10His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is >7.5 A650/cm/min/mg, as measured under the described conditions.
Animal Free,Carrier Free,Bioactive,ActiBioPure™,His-Tag,≥90%(SDS-PAGE) ActiBioPure™,Animal Free,Bioactive,Carrier Free,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Function This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
Specifications
Product Name
Recombinant Human P4HB Protein
Synonyms
Cellular thyroid hormone-binding protein | EC 5.3.4.1 | p55 | PDI | Prolyl 4-hydroxylase subunit beta | P4Hbeta | ERBA2L | CLCRP1 | thyroid hormone-binding protein p55 | Thbp | protocollagen hydroxylase | protein disulfide-isomerase | protein disulfide is
Grade
ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag
Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is >7.5 A650/cm/min/mg, as measured under the described conditions.
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20~-80℃ for more than 1 year. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Human P4HB Protein - Enzyme Activity Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is >7.5 A650/cm/min/mg, as measured under the described conditions. Materials: Buffer A: 100 mM NaH2PO4, 3 mM DTT, pH 7.0 Buffer B: 100 mM NaH2PO4, 3 mM EDTA, pH 7.0 Recombinant Human P4HB Protein (rp149672) Recombinant Human Insulin Protein (rp218456), 10 mg/mL solution in 0.01 M HCl Procedures： 1. Dilute insulin to 3.5 mg/mL in Buffer B. 2. Incubate diluted insulin for 10 minutes at room temperature (insulin will solubilize) 3. Dilute rhP4HB to 45 µg/mL in Buffer A. 4. Mix 100 µL of 45 µg/mL rhP4HB and 200 µL of 3.5 mg/mL insulin. Create a Substrate Blank with 100 µL Buffer A and 200 µL insulin. 5. Incubate at room temperature for 20 minutes. 6. Load in a clear 96-well plate 100 µL from each reaction vial in duplicate. 7. Read at 650 nm (absorbance) in kinetic mode for 5 minutes. 8. Calculate specific activity: *Adjusted for Substrate Blank **Note: the output of many spectrophotometers in kinetic mode is in mOD. ***Using the pathlength correction of 0.32 cm Per Well: rhP4HB: 0.0015 mg, Insulin: 0.100 mg.
Recombinant Human P4HB Protein (rp149672) - SDS-PAGE 2 μg/lane of Recombinant Human P4HB Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 55 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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