GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥100U/mg enzyme powder; ≥250U/mg protein
Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥100U/mg enzyme powder; ≥250U/mg protein ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 3 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Lactate oxidase catalyzes the oxidation of lactate to pyruvate and hydrogen peroxide in the presence of an FMN co-factor. The enzyme aids in the determination of L-lactate indirectly by measuring the hydrogen peroxide formed in the reaction.
Reaction
Product description
Appearance:
Yellow amorphous powder
Source:
Microorganism
Enzyme Commission Number:
EC 1.1.3.2
CAS Number:
9028-72-2
Storage temperature:
-20℃
Activity:
≥100U/mg enzyme powder; ≥250U/mg protein
Unit definition
One unit will convert one micromole of L-Lactate to pyruvate per min at pH 7.5 at 37℃.
Properties
Stability:
Stable at -20℃ for at least two years
Molecular weight:
40kDa (SDS-PAGE)
Isoelectric point:
5.2
Michaelis constant:
1.1×10⁻³M (L-Lactate)
Optimum pH:
6.5-8.0
{Fig. 1}
Optimum temperature:
37℃-40℃
{Fig. 3}
pH Stability:
5.0-10.0 (25℃, 20hr)
{Fig. 2}
Thermal stability:
< 50℃ (pH 7.0, 30min)
{Fig. 4}
Inhibitors:
Cu²⁺, Fe³⁺, Zn²⁺, SDS
Effect of various chemicals:
{Table 1}
Table 1. Effect of Various Chemicals on LOX
[The enzyme dissolved in 50mM Tris-HCl buffer, pH 7.5 (50U/ml) was incubated with each chemical at 37℃ for 2hr.]
Specifications
Product Name
Recombinant Lactate Oxidase (LOX), CAS No.9028-72-2
Synonyms
L-lactate oxidase
Grade
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
Specifications & Purity
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥100U/mg enzyme powder; ≥250U/mg protein
Bioactivity
≥100U/mg enzyme powder; ≥250U/mg protein
Predicted molecular weight
40 kDa
CAS
9028-72-2
Enzyme Commission Number
EC 1.1.3.2
Molecule Type
Enzyme
Storage and Shipping
Concentration
≥100U/mg enzyme powder; ≥250U/mg protein
Reconstitution
Reconstitute in water.
Storage
Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (2 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.
Unit definition
One unit will convert one micromole of L-Lactate to Pyruvate per minute at pH 7.5 at 37℃.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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