Tricine - PharmPure™, Low Endotoxin, ≥99% , CAS No.5704-04-1

CAS: 5704-04-1 Cat. No.: GMP1527622 Molecular Weight: 179.17 Beilstein Registry Number: 1937804 EC Number: 227-193-6 PubChem CID: 79784

GRADE & PURITY PharmPure™, Low Endotoxin, ≥99%

Storage

Room temperature

Shipped In

Normal

Overview

Tricine is an organic derivative of tris and glycine used in buffer solutions. It is a zwitterionic amino acid with a useful buffering range of pH 7.4-8.8 and is one of Good's buffering agents; Good first prepared tricine to buffer chloropast reactions. A zwitterionic amino acid with a buffering range of pH 7.4-8.8. Due to a low negative charge and a high ionic strength, Tricine is very useful for electrophoretic separation of small molecular weight proteins in the range of 1 to 100 kDa. Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a lower negative charge than glycine allowing it to migrate faster. In addition its high ionic strength causes more ion movement and less protein movement. This allows for low molecular weight proteins to be separated in lower percent acrylamide gels. Tricine has been documented in the separation of proteins in the range of 1 to 100 kDa by electrophoresis.[2] The tricine buffer at 25 mmol/L was found to be the most effective buffer among the ten tested for ATP assays using firefly luciferase.[3] Tricine has also been found to be an effective scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Buffer component for separation of low molecular weight peptides. A zwitterionic buffer useful in the pH range of 7.4-8.8. Has a pKa of 8.05 at 25°C.

A buffer component for separation of low molecular weight peptides.

Specifications

Specifications & Purity

PharmPure™, Low Endotoxin, ≥99%

Storage

Room temperature

Shipped In

Normal

Grade

Low Endotoxin, PharmPure™

Purity

≥99%

Names and Identifiers

• Canonical Smiles: C(C(=O)O)NC(CO)(CO)CO
• IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
• InChIKey: SEQKRHFRPICQDD-UHFFFAOYSA-N
• INCHI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• Isomeric SMILES: C(C(=O)O)NC(CO)(CO)CO
• WGK Germany: 3
• PubChem CID: 79784
• Molecular Weight: 179.17
• Beilstein: 1937804
• Reaxy-Rn: 1937804

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives
            • Level7 Alpha amino acids

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives - Alpha amino acids and derivatives
• Direct Parent: Alpha amino acids
• Alternative Parents: Amino acids 1,2-aminoalcohols Monocarboxylic acids and derivatives Dialkylamines Carboxylic acids Primary alcohols Organopnictogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: Alpha-amino acid - 1,2-aminoalcohol - Amino acid - Carboxylic acid - Secondary aliphatic amine - Monocarboxylic acid or derivatives - Secondary amine - Organic nitrogen compound - Primary alcohol - Organooxygen compound - Organonitrogen compound - Hydrocarbon derivative - Organic oxide - Organopnictogen compound - Carbonyl group - Amine - Organic oxygen compound - Alcohol - Aliphatic acyclic compound
• Description: This compound belongs to the class of organic compounds known as alpha amino acids. These are amino acids in which the amino group is attached to the carbon atom immediately adjacent to the carboxylate group (alpha carbon).
• External Descriptors: tricine

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in water (89.6 g/l) at 20 °C.
• Melt Point(°C): 172 °C
• Molecular Weight: 179.170 g/mol
• XLogP3: -5.100
• Hydrogen Bond Donor Count: 5
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 6
• Exact Mass: 179.079 Da
• Monoisotopic Mass: 179.079 Da
• Topological Polar Surface Area: 110.000 Ų
• Heavy Atom Count: 12
• Formal Charge: 0
• Complexity: 137.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Sun Yu-Long, Ge Bing-Qiang, Li Mi-Zhuan, Wang Lei, Chen Zhong-Xiu.  (2023)  The effect of macromolecular crowding degree on the self-assembly of fatty acid and lipid hydrolysis.  npj Science of Food,  7  (1): (1-8).  [PMID:37495579] [10.1038/s41538-023-00213-2]

2. Ziwei Su, Xiangxiang Zhang, Weichen Wang, Mingdong Dong, Xiaojun Han.  (2022)  Light-Harvesting Artificial Cells for the Generation of ATP and NADPH†.  CHINESE JOURNAL OF CHEMISTRY,  41  (1): (57-63).  [PMID:] [10.1002/cjoc.202200482]

3. Ping Sun, Jing Wu, Shenghong Yang, Hongli Li, Lei Zhao, Yuanhang Wang, Xiayan Wang, Qiaosheng Pu.  (2021)  Robust and easy-to-use microchip electrophoresis within sub-millimeter channels for fast and highly efficient separation.  TALANTA,      [PMID:34517615] [10.1016/j.talanta.2021.122747]

4. Yongjia Tang, Haoran Xu, Yaxue Dai, Fang Wang, Wenjing Huang, Peifei Liu, Yueqing Gu.  (2021)  A novel peptide targeting c-Met for hepatocellular carcinoma diagnosis.  Journal of Materials Chemistry B,  9  (22): (4577-4586).  [PMID:34047746] [10.1039/D1TB00408E]

5. Yuanbiao Tu, Ji Tao, Fang Wang, Peifei Liu, Zhihao Han, Zhaolun Li, Yi Ma, Yueqing Gu.  (2020)  A novel peptide targeting gastrin releasing peptide receptor for pancreatic neoplasm detection.  Biomaterials Science,  8  (9): (2682-2693).  [PMID:32266897] [10.1039/D0BM00162G]