α-1,4-Galactosyltransferase (LgtC), CAS No.52725-57-2

CAS: 52725-57-2 Cat. No.: G293642

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), expressed in E.coli;≥2U/mg protein; Protein concentration: See COA

Protein Tag

N-His

Expression system

E. coli

Bioactivity

≥2U/mg protein

Overview

α-1,4-Galactosyltransferase (LgtC), EC 2.4.1.228.The enzyme gene is derived from Neisseria meningitidis. It catalyzes the transfer of galactose onto glycan chains with galactose at the non‑reducing end. This product is recombinantly expressed in Escherichia coli, featuring high purity and high activity.

Reaction Equation

UDP‑Gal + β1‑4GalβGlcOR → α1‑4Galβ1‑4GalβGlcOR + UDP

Usage Instructions

Add substrates to the reaction system, along with 5 mM MgCl₂ and 1 mM DTT. Appropriate buffer salts (e.g., Tris‑HCl) may be supplemented to adjust the pH to 7.5, followed by addition of an appropriate amount of LgtC according to reaction requirements.

After mixing, incubate the sample at 37 °C for 2–4 hours.

Notes

For scaled‑up reactions, incubation time or enzyme dosage should be adjusted accordingly; low‑speed shaking is recommended for mixing.

Most common biological buffers (e.g., Tris or PBS) are suitable. Buffers outside this pH range (e.g., acetate buffer) may also be applicable, yet incubation time or enzyme dosage needs optimization based on practical conditions.

Precautions

For your safety and health, wear a lab coat and disposable gloves during operation. This product is for research use only.

Specifications

Product Name

α-1,4-Galactosyltransferase (LgtC), CAS No.52725-57-2

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), expressed in E.coli;≥2U/mg protein; Protein concentration: See COA

Bioactivity

≥2U/mg protein

Predicted molecular weight

32 kDa

CAS

52725-57-2

Enzyme Commission Number

EC 2.4.1.228

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in E.coli;≥2U/mg protein; Protein concentration: See COA

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

One unit is defined as the amount of enzyme required to transfer 1 μmol of galactose (Gal) from UDP‑Gal to lactose to form Galα1,4‑lac per minute at 37 °C.