5(6)-Aminofluorescein - for fluorescence analysis, ≥94%(HPLC) , CAS No.27599-63-9

CAS: 27599-63-9 Cat. No.: A107604 Molecular Weight: 347.32 Beilstein Registry Number: 6538782 PubChem CID: 76845

GRADE & PURITY for Fluorescence analysis, ≥94%(HPLC)

Synonyms

Fluoresceinamine, isomer 1 | Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 5-amino-3',6'-dihydroxy- | fluorescein-5-amine | EX-A1779 | Fluoresceinamine isomer 1 | SCHEMBL220211 | 5-Amino-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-(9H)xanthen]-3-one |

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Specifications

Synonyms

Fluoresceinamine, isomer 1 | Spiro(isobenzofuran-1(3H), 9'-(9H)xanthen)-3-one, 5-amino-3', 6'-dihydroxy- | fluorescein-5-amine | EX-A1779 | Fluoresceinamine isomer 1 | SCHEMBL220211 | 5-Amino-3', 6'-dihydroxyspiro[isobenzofuran-1(3H), 9'-(9H)xanthen]-3-one |

Specifications & Purity

for fluorescence analysis, ≥94%(HPLC)

Biochemical and Physiological Mechanisms

Two isomers are produced by the usual synthesis. Most publications imply they are interchangeable when used for the preparation of the isothiocyanate for fluorescent labeling.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

for Fluorescence analysis

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Purity

≥94%(HPLC)

Names and Identifiers

• Pubchem Sid: 488185336
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/488185336
• Canonical Smiles: Oc1ccc2c(c1)Oc1cc(O)ccc1C12OC(=O)c2cc(N)ccc12
• Isomeric SMILES: C1=CC2=C(C=C1N)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
• WGK Germany: 3
• PubChem CID: 76845
• Molecular Weight: 347.32
• Beilstein: 6538782

Chemical and Physical Properties

• Molecular Weight: 347.300 g/mol
• XLogP3: 2.700
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 0
• Exact Mass: 347.079 Da
• Monoisotopic Mass: 347.079 Da
• Topological Polar Surface Area: 102.000 Ų
• Heavy Atom Count: 26
• Formal Charge: 0
• Complexity: 557.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Liu Yipeng, Li Bingyan, Zhang Huixia, Liu Yong, Xie Ping.  (2022)  Detection of Microcystin-LR in the Cells and Natural Lake Water Samples by A Unique Fluorescence-Based Method.  JOURNAL OF FLUORESCENCE,  32  (2): (505-519).  [PMID:34981282] [10.1007/s10895-021-02882-2]

2. Dongxu Lan, Yulian Shang, Hongxian Su, Minhua Liang, Yang Liu, Haofei Li, Qi Feng, Xiaodong Cao, Hua Dong.  (2021)  Facile Fabrication of Hollow Hydrogel Microfiber via 3D Printing-Assisted Microfluidics and Its Application as a Biomimetic Blood Capillary.  ACS Biomaterials Science & Engineering,      [PMID:34503336] [10.1021/acsbiomaterials.1c00980]

3. Li Liu, Ying Shi, Menglu Li, Chaoqun Sun, Yijuan Long, Huzhi Zheng.  (2017)  Effect of carboxyl and amino groups in fluorescein molecules on their peroxidase-like activity.  Molecular Catalysis,      [PMID:] [10.1016/j.mcat.2017.06.029]

4. Wenhua Li, Yurong Cai, Qiwei Zhong, Ying Yang, Subhas C. Kundu, Juming Yao.  (2015)  Silk sericin microcapsules with hydroxyapatite shells: protection and modification of organic microcapsules by biomimetic mineralization.  Journal of Materials Chemistry B,  4  (2): (340-347).  [PMID:32263376] [10.1039/C5TB02328A]

5. Qinghua Xie, Xuemei Wang, Gaorui Zhang, Dawei Zhou, Yuxuan Zhao, Hong Liu, Jiazhi Duan, Dexin Yu, Yuanhua Sang.  (2024)  Ultrasmall Fe3O4 nanoparticles self-assembly induced dual-mode T1/T2-weighted magnetic resonance imaging and enhanced tumor synergetic theranostics..  Scientific Reports,  14  (1): (10646-10646).  [PMID:38724530] [10.1038/s41598-024-59525-2]