Acridine Orange hemi(zinc chloride) salt - for electrophoresis , CAS No.10127-02-3

CAS: 10127-02-3 Cat. No.: A100495 Molecular Weight: 369.96 Beilstein Registry Number: 3734978 EC Number: 233-353-6 PubChem CID: 159263

GRADE & PURITY Suitable for electrophoresis

Synonyms

C.I. 46005 | Euchrysin GNY | Euchrysine 3RX | AKOS027250830 | N,N,N inverted exclamation marka,N inverted exclamation marka-Tetramethylacridine-3,6-diamine monohydrochloride compd. with zinc dichloride | BasicOrange14 | DTXSID20906035 | N,N,N',N'-Tetramet

Storage

Store at 2-8°C,Argon charged

Shipped In

Wet ice

Overview

Acridine Orange hemi (zinc chloride) salt is a cell-permeable metachromatic fluorescent dye that stains DNA and RNA. This dye is also an inhibitor of RNA polymerase.
Fluorescent dye that stains DNA and RNA

Application

Suitable for

detection of nucleic acids separated by gel electrophoresis

fluorescence and epifluorescence microscopy

analysis of mitochondria and lysosomes by flow cytometry

DNA staining in apoptosis studies

Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes.

Specifications

Synonyms

C.I. 46005 | Euchrysin GNY | Euchrysine 3RX | AKOS027250830 | N, N, N inverted exclamation marka, N inverted exclamation marka-Tetramethylacridine-3, 6-diamine monohydrochloride compd. with zinc dichloride | BasicOrange14 | DTXSID20906035 | N, N, N', N'-Tetramet

Specifications & Purity

for electrophoresis

Biochemical and Physiological Mechanisms

Cell-permeable metachromatic fluorescent cationic dye that stains DNA and RNA. Also an inhibitor of RNA polymerase.

Storage

Store at 2-8°C, Argon charged

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Suitable for electrophoresis

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Toxic, refer to SDS for further information . Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Names and Identifiers

• Canonical Smiles: CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C.Cl.[Cl-].[Cl-].[Zn+2]
• IUPAC Name: zinc;3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine;dichloride;hydrochloride
• InChIKey: VADJQOXWNSPOQA-UHFFFAOYSA-L
• INCHI: 1S/C17H19N3.3ClH.Zn/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;;;/h5-11H,1-4H3;3*1H;/q;;;;+2/p-2
• Isomeric SMILES: CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C.Cl.[Cl-].[Cl-].[Zn+2]
• WGK Germany: 3
• PubChem CID: 159263
• Molecular Weight: 369.96
• Beilstein: 3734978
• Reaxy-Rn: 10510633

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Quinolines and derivatives
      • Subclass Benzoquinolines

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Quinolines and derivatives
• Subclass: Benzoquinolines
• Intermediate Tree Nodes: Not available
• Direct Parent: Acridines
• Alternative Parents: Aminoquinolines and derivatives Dialkylarylamines Pyridines and derivatives Benzenoids Heteroaromatic compounds Organic transition metal salts Organic metal halides Azacyclic compounds Organopnictogen compounds Organic chloride salts Hydrochlorides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Acridine - Aminoquinoline - Dialkylarylamine - Tertiary aliphatic/aromatic amine - Benzenoid - Pyridine - Heteroaromatic compound - Tertiary amine - Organic metal halide - Azacycle - Organic transition metal salt - Organic nitrogen compound - Organopnictogen compound - Hydrocarbon derivative - Organic chloride salt - Hydrochloride - Organic salt - Organonitrogen compound - Amine - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as acridines. These are organic compounds containing the acridine moiety, a linear tricyclic heterocycle which consists of two benzene rings joined by a pyridine ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in water (1 mg/ml).
• Sensitivity: Moisture sensitive.
• Molecular Weight: 438.100 g/mol
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 2
• Exact Mass: 435.001 Da
• Monoisotopic Mass: 435.001 Da
• Topological Polar Surface Area: 19.400 Ų
• Heavy Atom Count: 24
• Formal Charge: 0
• Complexity: 298.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 5

XLogP3

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