GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.for Protein analysis?Protein-analysis grade — low-interference reagents for protein quantitation/characterization. Use in protein assays where purity affects accuracy.for Protein sequencing?Protein-sequencing grade — ultra-clean reagents for Edman/MS sequencing. Use where contaminants would obscure sequence determination.
BioReagent,for protein analysis,for protein sequencing BioReagent,for Protein analysis,for Protein sequencing for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Room temperature Ships Normal Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Amido Black 10B can be used to detect proteins on blotting membranes. When the transferred protein is greater than 50 ng per band, dark blue bands appear on a light blue background. Amido Black is the most commonly used stain for protein sequencing and in situ protein cleavage when determining internal sequences.
Amido Black 10B Staining Washing Solution is primarily composed of methanol, weak acid, deionized water, etc. It is suitable for destaining after Amido Black 10B staining on cellulose acetate membranes. This reagent is for research use only and is not intended for clinical diagnosis or other purposes.
Materials to Prepare
Distilled or deionized water
Amido Black 10B Staining Solution
Shaker
Procedure (For Reference Only)
Place the blotting transfer membrane in a suitable container. Wash with distilled water 3 times, 5 minutes each time.
Immerse the membrane in Amido Black 10B Staining Solution (0.1%) for 5 minutes or longer. Shake the staining dish occasionally during the staining process.
Prepare 3 destaining dishes containing Amido Black 10B Staining Washing Solution. Remove the membrane strip from the staining solution, drain off as much staining solution as possible, and successively immerse it in the washing solutions. Rinse repeatedly until the background is colorless.
Appropriately record or photograph the results.
Precautions
For your safety and health, please wear a lab coat and disposable gloves during operation.
Use the reagent as soon as possible after opening to prevent affecting subsequent experimental results.
Specifications
Specifications & Purity
BioReagent, for protein analysis, for protein sequencing
Stability And Storage
Store at room temperature long term (12 months).
Storage
Room temperature
Shipped In
Normal
Grade
BioReagent, for Protein analysis, for Protein sequencing
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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