C4b-Binding Protein from Human Plasma

Cat. No.: np001118

GRADE & PURITY BioReagent, ≥95%(SDS-PAGE), Pre-lyophilization Protein Concentration

Accession #

P20851,P04003

Overview

C4b-binding protein is a regulatory protein of the Complement Cascade that possesses decay-accelerating activity. Circulating in plasma, it aids in the control of fluid-phase and surface-associated activation of C3 and C5 and serves as a cofactor to Factor 1 in the proteolytic degradation of C4b.
There are three isoforms circulating in plasma; 7 alpha chains and 1 Beta chain, 6 alpha chains and 1 Beta chain, and 7 alpha chains without a Beta chain. The 7 alpha, 1 Beta form is the most abundant. All C4BP molecules that contain a Beta chain circulate in plasma in a high-affinity, calcium-dependent complex with vitamin K-dependent, anticoagulant Protein S.
C4b-binding protein has been investigated for its role in Alzheimer's Disease, Psoriasis, as well as autoimmune diseases including Rheumatoid Arthritis, and different types of Cancer.
Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.
Free of Protein S.

Specifications

Product Name

C4b-Binding Protein from Human Plasma

Synonyms

C4b-binding protein alpha chain | C4bp | C4bpa | C4BPA_HUMAN | Complement component 4 binding protein | Complement component 4 binding protein alpha | Proline-rich protein | PrP | C4b binding protein beta chain | C4b-binding protein beta chain | C4BPB | C

Grade

BioReagent

Specifications & Purity

BioReagent, ≥95%(SDS-PAGE), Pre-lyophilization Protein Concentration

Biochemical and Physiological Mechanisms

C4BPA: Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissoci

Accession #

P20851, P04003

Predicted molecular weight

~570 kDa (whole molecule); ~70-75 kDa (alpha chains); ~40-45 kDa (beta chain)

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized from 10 mM Tris pH 7.4, 100 mM NaCl, 0.05% NaN3
\r\nFree of Protein S

Concentration

Pre-lyophilization Protein Concentration

Reconstitution

After centrifugation, we suggest addition of de-ionized H2O to the original volume (See COA), followed by gentle mixingto ensure adequate homogenization. If further dilution is required, please use the lyophilization buffer.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (2 years). Upon reconstitution, it is recommended to aliquot, store at ≤ -70℃ long term. Avoid freeze/thaw cycle.