Fast Green FCF - Biological stain , CAS No.2353-45-9

CAS: 2353-45-9 Cat. No.: F110919 Molecular Weight: 808.85 Beilstein Registry Number: 5718212 EC Number: 219-091-5

GRADE & PURITY Biological Stain

Synonyms

disodium 2-[(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)[(1E,4E)-4-{ethyl[(3-sulfonatophenyl)methyl]iminiumyl}cyclohexa-2,5-dien-1-ylidene]methyl]-5-hydroxybenzene-1-sulfonate | D90631 | FAST GREEN FCF [MI] | Benzenemethanaminium,N-ethyl-N-[4-[[4-[et

Storage

Room temperature,Argon charged,Desiccated,Cool

Shipped In

Normal

Overview

Fast Green FCF has been a widely utilized tissue and protein stain used in IEF and SDS-PAGE. Has the ability to determine efficiency of transfer and localization of sample proteins on blotting membranes. Other applications of Fast Green FCF include using its clastogenic properties and it may also act at a presynaptic locus inhibiting neurotransmitter release in whole-cell voltage clamped hippocampal interneurons. Has been shown to be a beneficial stain to detail integumental injuries in Chinook salmon. When bound to protein, Fast Green FCF fluoresces near infrared; color index #42053.
A widely utilized tissue and protein stain used in IEF & SDS-PAGE, fluoresces near IR

Specifications

Synonyms

disodium 2-[(4-{ethyl[(3-sulfonatophenyl)methyl]amino}phenyl)[(1E, 4E)-4-{ethyl[(3-sulfonatophenyl)methyl]iminiumyl}cyclohexa-2, 5-dien-1-ylidene]methyl]-5-hydroxybenzene-1-sulfonate | D90631 | FAST GREEN FCF [MI] | Benzenemethanaminium, N-ethyl-N-[4-[[4-[et

Specifications & Purity

Biological stain

Biochemical and Physiological Mechanisms

Widely utilized tissue and protein stain. Used as a quantitative stain for histones at alkaline pH after acid extraction of DNA and as a protein stain in IEF (isoelectric focusing) and SDS-PAGE electrophoresis. Fluoresces in the

Storage

Room temperature, Argon charged, Desiccated, Cool

Shipped In

Normal

Grade

Biological Stain

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Names and Identifiers

• Pubchem Sid: 504752786
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504752786
• Canonical Smiles: CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=C(C=C(C=C5)O)S(=O)(=O)[O-].[Na+].[Na+]
• IUPAC Name: disodium;2-[[4-[ethyl-[(3-sulfonatophenyl)methyl]amino]phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]methyl]-5-hydroxybenzenesulfonate
• InChIKey: RZSYLLSAWYUBPE-UHFFFAOYSA-L
• INCHI: 1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
• Isomeric SMILES: CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=C(C=C(C=C5)O)S(=O)(=O)[O-].[Na+].[Na+]
• WGK Germany: 2
• RTECS: BQ4425000
• Molecular Weight: 808.85
• Beilstein: 5718212
• Reaxy-Rn: 5718212
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=5718212&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • Class Benzene and substituted derivatives
      • Subclass Phenylmethylamines

• Kingdom: Organic compounds
• Superclass: Benzenoids
• Class: Benzene and substituted derivatives
• Subclass: Phenylmethylamines
• Intermediate Tree Nodes: Not available
• Direct Parent: Phenylbenzamines
• Alternative Parents: Diphenylmethanes Benzenesulfonic acids and derivatives 1-sulfo,2-unsubstituted aromatic compounds Benzenesulfonyl compounds Aniline and substituted anilines Benzylamines Dialkylarylamines 1-hydroxy-2-unsubstituted benzenoids Aralkylamines Sulfonyls Secondary ketimines Organosulfonic acids Azomethines Propargyl-type 1,3-dipolar organic compounds Organopnictogen compounds Organooxygen compounds Organic sodium salts Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic homomonocyclic compounds
• Substituents: Phenylbenzamine - Diphenylmethane - Benzenesulfonate - Benzenesulfonyl group - Arylsulfonic acid or derivatives - 1-sulfo,2-unsubstituted aromatic compound - Benzylamine - Tertiary aliphatic/aromatic amine - Dialkylarylamine - Aniline or substituted anilines - 1-hydroxy-2-unsubstituted benzenoid - Phenol - Aralkylamine - Organic sulfonic acid or derivatives - Organosulfonic acid - Organosulfonic acid or derivatives - Azomethine - Sulfonyl - Secondary ketimine - Tertiary amine - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Organic alkali metal salt - Amine - Organic salt - Organic sodium salt - Hydrocarbon derivative - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Organic oxide - Organopnictogen compound - Organic oxygen compound - Organic nitrogen compound - Aromatic homomonocyclic compound
• Description: This compound belongs to the class of organic compounds known as phenylbenzamines. These are aromatic compounds consisting of a benzyl group that is N-linked to a benzamine.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in water (150 g/l) at 25 °C, glycerin, glycol, alcohol, and ethanol.
• Sensitivity: Moisture sensitive
• Melt Point(°C): 290°C
• Molecular Weight: 808.900 g/mol
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 11
• Rotatable Bond Count: 9
• Exact Mass: 808.117 Da
• Monoisotopic Mass: 808.117 Da
• Topological Polar Surface Area: 223.000 Ų
• Heavy Atom Count: 54
• Formal Charge: 0
• Complexity: 1540.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 3

XLogP3

References

1. Chan Guo, Jian Lei, Wenjing Geng, Jiayao Lin, Sugang Meng, Sheng Ye, Yujun Zhu, Weiwei Lei, Caijin Huang, Jun Hu, Cheng Chen.  (2023)  Efficient pollutant removal using tetrahydrofuran functionalized carbon nitride nanosheets with enhanced photocatalytic performance.  APPLIED SURFACE SCIENCE,      [PMID:] [10.1016/j.apsusc.2023.159155]

2. Wenjing Geng, Zechen Zhang, Qi Guo, Yuchen Liu, Xinxin Ye, Hui Zhang, Chen Fu, Fengguang Liu, Yujun Zhu, Cheng Chen.  (2023)  Tailoring graphene oxide nanofiltration membrane with adjustable nanochannels for enhanced molecule separation.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2023.147327]

3. Mao Xu, Qi Tang, Yuchen Liu, Jiaqi Shi, Weiyu Zhang, Chan Guo, Qiuwen Liu, Weiwei Lei, Cheng Chen.  (2023)  Charged Boron Nitride Nanosheet Membranes for Improved Organic Solvent Nanofiltration.  ACS Applied Materials & Interfaces,      [PMID:36820819] [10.1021/acsami.2c20893]

4. Hongli Chang, Weihong Ji, Yule Xie, Shujun He, Zhenfeng Xie, Fengjie Sun.  (2023)  Morphological Characterization of Metamorphosis in Stamens of Anemone barbulata Turcz. (Ranunculaceae).  Agronomy-Basel,  13  (2): (554).  [PMID:] [10.3390/agronomy13020554]

5. Chunling Nie, Xianpan Bu, Sihong Ma, Jiahui Zhang, Qin Ma, Weilong Li, Xiaowan Zhang, Hao Wu, Shuqian Hu, Guangsen Fan, Jianguo Wang.  (2022)  Pickering emulsions synergistically stabilized by cellulose nanocrystals and peanut protein isolate.  LWT-FOOD SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.lwt.2022.113884]

6. Shuangshou Wang, Lu Zhang, Qiwen Jin, Zhongqiu Xu, Jiayi Zhao, Yuwen Ding, Wenzhi Li, Peng Lin, Jing Gu, Qi Zhang, Yang Chen, Hongmei Chen, Tingxuan Yan.  (2022)  Filter paper-based colorimetric analysis: An instrument-free strategy for semiquantitative naked-eye detection of food colorants.  FOOD CHEMISTRY,      [PMID:35551021] [10.1016/j.foodchem.2022.133087]

7. Shuangshou Wang, Wenzhi Li, Zhihong Yuan, Qiwen Jin, Zongpeng Ding, Xiaomeng Liu, Mingfu Ye, Jing Gu, Tingxuan Yan, Hongmei Chen, Yang Chen.  (2022)  Semiquantitative naked-eye detection of synthetic food colorants using highly-branched pipette tip as an all-in-one device.  ANALYTICA CHIMICA ACTA,      [PMID:35589223] [10.1016/j.aca.2022.339901]

8. Ningning Ouyang, Haile Ma, Yanhua Ding, Feng Lu, Lina Guo, Xueli Zhang, Chen Gu.  (2021)  Effect of slit dual-frequency ultrasonic emulsification technology on the stability of walnut emulsions.  ULTRASONICS SONOCHEMISTRY,      [PMID:34942470] [10.1016/j.ultsonch.2021.105876]

9. Yaru Xing, Yu Liu, Rifei Chen, Yuyan Li, Chengzhi Zhang, Youwei Jiang, Yao Lu, Bingcheng Lin, Peizhong Chen, Ruijun Tian, Xianming Liu, Xing Cheng.  (2021)  A robust and scalable active-matrix driven digital microfluidic platform based on printed-circuit board technology.  LAB ON A CHIP,  21  (10): (1886-1896).  [PMID:34008645] [10.1039/D1LC00101A]

10. Sun Qiangqiang, Wang Dehui, Li Yanan, Zhang Jiahui, Ye Shuji, Cui Jiaxi, Chen Longquan, Wang Zuankai, Butt Hans-Jürgen, Vollmer Doris, Deng Xu.  (2019)  Surface charge printing for programmed droplet transport.  NATURE MATERIALS,  18  (9): (936-941).  [PMID:31332340] [10.1038/s41563-019-0440-2]

11. Tian-Dan Lu, Bo-Zhi Chen, Jue Wang, Tian-Zhi Jia, Xue-Li Cao, Yong Wang, Weihong Xing, Cher Hon Lau, Shi-Peng Sun.  (2018)  Electrospun nanofiber substrates that enhance polar solvent separation from organic compounds in thin-film composites.  Journal of Materials Chemistry A,  6  (31): (15047-15056).  [PMID:] [10.1039/C8TA04504F]

12. Shuangshou Wang, Jianing Hui, Yangrui Si, Tianyou Geng, Chunyan Yang, Yanshang Kang, Daojin Li, Mingfu Ye, Tingxuan Yan, Caibo Yue.  (2025)  Deep-eutectic solvent coated paper: A reusable multi-purpose device for rapid visualization, adulteration identification, efficient removal and recycling of dyes in both food and environmental samples.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2024.112625]

13. Chang Liu, Pan-Pan Tang, Xiu-Bin Liu, Najla AlMasoud, Taghrid S. Alomar, Rana Muhammad Aadil, Jun-Hu Cheng, Zhi-Wei Liu.  (2025)  Cold plasma-induced ovalbumin amyloid fibrils: Morphological characteristics and stability on astaxanthin-loaded high internal phase emulsions.  Food Chemistry-X,      [PMID:40823133] [10.1016/j.fochx.2025.102835]

14. Liu Nan-nan, Ren Qing-dan, Li Long, Wang Li-na, Shi Guang-liang, Qin Shou-tao.  (2025)  Multi-omics analysis of the characteristic components of Apis Cerana honey from Changbai mountain.  EUROPEAN FOOD RESEARCH AND TECHNOLOGY,      [PMID:] [10.1007/s00217-025-04903-x]

15. Yanduo Zhai, Yande Cui, Meng Zhou, Pan Chen, Chunyu Chang, Jinghua Chen.  (2024)  Large-scale additive fabrication of tough nanocellulose yarns.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2024.154105]

16. Jing Gu, Lu Zhang, Yangrui Si, Shuang Li, Jianzu Shen, Ruohan Xu, Tingxuan Yan, Mingfu Ye, Daojin Li, Qiang Wang, Changfang Zhang, Shuangshou Wang.  (2025)  Colorimetry collaborated with WeChat applet: An on-screen direct-reading solution for end-to-end real-time monitoring of adsorption, degradation and recycle of dyes in water environment.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2025.114965]

17. Xuewei Zhang, Zhihao Fang, Boon Chin Heng, Xiaoyu Hu, Zigang Ge.  (2025)  Mitigating Oxidative Stress Enhances Cartilage Regeneration by Ameliorating Apoptosis of Cartilage Progenitor Cells in Adult Mice.  Advanced Biology,      [PMID:40110588] [10.1002/adbi.202400739]

18. Pengrui Wu, Chunling Nie, Zhihong Song, Xindi Wei, Yefan Niu, Mehdi Tabarsa, Li Wang, Jianguo Wang.  (2025)  Improve the bioaccessibility of curcumin encapsulated in Pickering emulsions stabilized by double fibrils.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,      [PMID:] [10.1016/j.colsurfa.2025.137212]

19. Mengzhe Li, Xin Wang, Tong Shi, Xiaoli Zhang, Wengang Jin, Li Yuan, Ruichang Gao.  (2025)  Effects of oxidation on the emulsion properties of snakehead (Channa argus) myofibrillar protein: Roles of structural and interfacial changes.  FOOD CHEMISTRY,      [PMID:41130007] [10.1016/j.foodchem.2025.146770]

20. Chengmei Bai, Qian Ye, Ling Wang, Bin Li, Shilin Liu, Yan Li.  (2025)  Demulsification of water-in-oil emulsions stabilized with glycerol monostearate-based oleogel by secondary surfactant to enhance saltiness perception.  FOOD HYDROCOLLOIDS,      [PMID:] [10.1016/j.foodhyd.2025.112224]

21. Xue-Ting Zhai, Xiao-Wei Wu, Fangang Meng, Yuanyuan Zhang, Yu-Xi Huang.  (2025)  Fabrication of MAF-6-based nanofiltration membranes and their application in dye/salt separation.  DESALINATION,      [PMID:] [10.1016/j.desal.2025.119671]

22. Yi Dai, Jianyi Xu, Jie Hao, Zhang Liu, Chengze Lan, Guanghua Xu, Xizhi Meng, Yating Deng, Peng Wan, Kunxing Liu, Yingtao Yao, Fang Zhao, Peng Fei, Xiu Nie, Gang Lv, Dan Zhu, Tingting Yu.  (2025)  Chemical dehydration-induced ultrafast clearing method for human tissues.  VIEW,      [PMID:] [10.1002/VIW.20250104]

23. Yuying Hu, Qian Liu, Jiaqi Duan, Yihang Yang, Yanhong Li, Dongli Fu, David Julian McClements, Bin Zhou.  (2026)  Tailoring the properties of egg white protein-based emulsion gels for 3D printing: Role of droplet size and matrix concentration.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:41611146] [10.1016/j.ijbiomac.2026.150569]

24. Yuying Hu, Jiaqi Duan, Yihang Yang, Yanhong Li, Dongli Fu, Qian Liu, David Julian McClements, Bin Zhou.  (2026)  Exploring the gastrointestinal fate of composite emulsion gels as food models: Impact of texture and oral processing on lipid digestion.  FOOD CHEMISTRY,      [PMID:] [10.1016/j.foodchem.2026.148708]