GFP/EGFP Mouse mAb (Biotin) - Primary antibody, specific to Green fluorescent protein

Cat. No.: Ab220741
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. 0.5 mg/mL
Application
WB
Host species
Mouse
Reactive species
Species independent
Isotype
Mouse IgG1
Clone Number
IJ03/2E8
Conjugation
Biotin
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab220741-10μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$25.90
50μl
Ab220741-50μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$75.90
100μl
Ab220741-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$119.90
1ml
Ab220741-1ml
8-12 wks(?) Production requires sourcing of materials. We appreciate your patience and understanding.
$699.90
Enter a quantity for the sizes you want to add.
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Why this grade

Azide Free,ExactAb™,Validated,0.5 mg/mL Azide Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti GFP/EGFP Antibody, clone IJ03/2E8, could be used for WB and so on.

Specifications

Product Name
GFP/EGFP Mouse mAb (Biotin) - Primary antibody, specific to Green fluorescent protein
Synonyms
GFP antibody | Green fluorescent protein antibody | Anti-Green Fluorescent Protein (GFP) Antibody | Mouse anti-GFP/eGFP-tag Monoclonal | Monoclonal Anti-GFP antibody produced in mouse | GFP Mouse Monoclonal Antibody | GFP-Tag Monoclonal Antibody | GFP ant
Specifications & Purity
Azide Free, ExactAb™, Validated, 0.5 mg/mL
Host species
Mouse
Specificity
Green fluorescent protein
Immunogen
Recombinant full length protein corresponding to Aequorea Victoria GFP.
Positive Control
WB: HEK293 cells transfected with eGFP tags.
Conjugation
Biotin
Grade
Azide Free, ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Images
GFP/EGFP Mouse mAb (Biotin) (Ab220741) - Western Blot
All lanes: GFP/EGFP Mouse mAb (Biotin) (Ab220741) at 1/10000 dilution 
Samples: Lysates at 20 µg per lane 
Secondary: SA-HRP (np156148) at 1/20000 dilution Predicted band size: 48 kDa 
Observed band size: 51 kDa Exposure time: 12.1 s
Application
ApplicationDilution info
WB

1/1000 - 1/10000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide →

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