GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Mouse MHC class I/H-2Kb Antibody, Monoclonal (AF6-88.5.5.3), could be used for Flow Cytometry.
Specifications
Product Name
MHC class I/H-2Kb Mouse mAb (AF700) - Primary antibody, specific to MHC class I/H-2Kb
Synonyms
K-f | H-2K | H2-K | H2-D1 | H-2K(d) | A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Host species
Mouse
Specificity
MHC class I/H-2Kb
Immunogen
C57BL/6 mouse splenocytes
Positive Control
Flow Cytometry: C57BL/6 mouse splenocytes.
Conjugation
AF700
Excitation (nm)
Red Laser(633nm)
Emission(nm)
719nm
Ex/Em(nm)
Ex:696nm;Em:719nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
MHC class I/H-2Kb Mouse mAb (AF700) (Ab190298) - Flow Cytometry Flow cytometry analysis of MHC class I/H-2Kb expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with MHC class I/H-2Kb Mouse mAb (AF700) (Ab190298) at 0.3μg/test for 1 hour at 4°C. Left panel - Mouse IgG (AF700) (Ab190248).
MHC class I/H-2Kb Mouse mAb (AF700) (Ab190298) - Flow Cytometry Flow cytometry analysis of MHC class I/H-2Kb expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with MHC class I/H-2Kb Mouse mAb (AF700) (Ab190298) at 0.3μg/test for 1 hour at 4°C. Blue - Mouse IgG (AF700) (Ab190248). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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