Mouse β-NGF ELISA Kit

Cat. No.: M1509896

GRADE & PURITY BioReagent

Overview

This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Mouse β-NGF concentration in samples. The Mouse β-NGF capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Mouse β-NGF present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Mouse β-NGF is added. This antibody binds to the captured Mouse β-NGF, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Mouse β-NGF is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofMouse β-NGF is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofMouse β-NGF, and the concentration of Mouse β-NGF in unknown samples is interpolated from this curve based on their OD values.

Storage and Shipping

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ for 6 months.