Specific Activity ＞250 U/mg;Activity 15 U/ml for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Product Description
Sialidase, N-acetylneuraminate glycohydrolase, Exo-alpha-sialidase
α(2-3,6) Neuraminidase Cp cleaves all non- reducing terminal non-branched &alpha(2-3)- and α(2-6) sialic acid residues from complex carbohydrates and glycoproteins. There is no detectable activity on α (2-8) or α(2-9) linkages or on branched α(2-3) or α(2-6) linkages. The relative cleavage rates for different linkages are: α(2-3) > α(2-6).
α(2-3,6) Neuraminidase Cp will not cleave branched sialic acids (linked to an internal residue). Use α(2-3,6,8,9) Neuraminidase for α(2-8) or branched sialic acids. To cleave only non-reducing terminal α(2-3) unbranched sialic acid residues, use α(2-3) Neuraminidase.
α(2-3,6) Neuraminidase Cp is isolated from a clone of Clostridium perfringens. The enzyme has been extensively characterized using oligosaccharide standards.
Specifictity Cleaves all nonreducing terminal non-branched alpha-(2-3) and alpha-(2-6)-sialic acid residues from complex carbohydrates and glycoproteins.
Relative cleavage rates for different linkages are: (2-3) > (2-6).
Source recombinant from Clostridium perfringens in E. Coli.
EC 3.2.1.18
Specific Activity >250 U/mg
Activity 15 U/ml
Molecular weight 41,000 daltons
pH range 50 mM sodium phosphate (pH 6.0) provides the optimal buffer for enzyme activity with 3’-siayllactose, a standard substrate. If glycosidase treatment is performed at suboptimal pH because of glycoprotein solubility or activity requirements, expect some diminution in enzyme activity.
Specific Activity Assay Defined as the amount of enzyme required to produce 1 µmole of methylumbelliferone in 1 minute at 37˚C, pH 5.0 from MU-NANA [2′-(4-methylumbelliferyl)-alpha-D-N-acetylneuraminic acid].
Contents
Neuraminidase Cp in 20 mM Tris-HCl, 25 mM NaCl, pH 7.5
Included its 20 µL and 60 µL pack sizes:
5x Reaction Buffer 250 mM sodium phosphate, pH 6.0
Suggested usage
1. Add up to 100 μg of glycoprotein or 1 nmol of oligosaccharide to tube.
2. Add water to 14 μL
3. Add 4 μL 5X Reaction Buffer.
4. Add 2 μL α (2-3,6) Neuraminidase.
5. Incubate at 37°C for 1 hour.
Desialylation may be monitored by SDS-PAGE if the size differential between native and desialylated protein is sufficient for detection.
Storage
Store enzyme at 4˚C.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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