Arachis hypogaea lectin or Peanut Agglutinin (PNA) is isolated from peanuts and purified by affinity chromatography. The lectin has a molecular weight of 110 kDa and consists of four identical subunits of approximately 27 kDa each.
PNA is a carbohydrate-free protein that displays specificity towards β-D-Gal(1-3)-D-GalNAc. It has potent anti-T activity and can be used to distinguish between human lymphocyte subsets. PNA has been used in tumour tissue determination for transitional mucosa malignancies. The lectin agglutinates rabbit erythrocytes at < 0.1 μg/ml after trypsin treatment of cells and its activity is inhibited by lactose and galactose.
Aladdin’s PNA lectin is provided as a white to light-yellow lyophilized powder from 10 mM NH₄HCO₃. The purity is determined by SDS-PAGE, which generates one major band at 25-27 kDa.
Benefits
• Strong anti-T activity
• Sugar specificity: β-D-Gal-(1-3)-D-GalNAc
• Agglutinates rabbit erythrocytes at < 0.1 μg/ml after trypsin treatment of the cells
• High activity
Applications
• Probe in histochemistry and immuno-histochemistry
• Binds to a broad range of receptors in human tissues
• Human lymphocyte subset studies
Specifications
| Parameter | Details |
|---|
| Appearance | White to light-yellow lyophilized powder | | Source | Peanuts | | Molecular weight | 110 kDa | | Sugar specificity | β-D-Gal-(1-3)-D-GalNAc | | Activity | Agglutinates rabbit erythrocytes at < 0.1 μg/ml. Agglutination fully inhibited by 10 mM D-galactose | | Microorganisms | ≤ 100 CFU/g | | Shelf life | ≥ Three years when stored at -20°C |
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Directions for use
The lectin may be reconstituted with PBS or the buffer of your choice before use. Spin the vial until dissolved.
Tips and hints
Avoid repeated freezing and thawing.
Shipping and storage
The product is stable for at least 3 years from production date when stored below -20°C. May be shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.
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