Thionin acetate - Dye content 85 % , CAS No.78338-22-4

CAS: 78338-22-4 Cat. No.: T304644 Molecular Weight: 287.34 EC Number: 676-854-9 PubChem CID: 2724414

GRADE & PURITY Dye content 85 %

Synonyms

Thioninacetat | Thionin acetate (Thionine acetate) | Thionin acetate salt, certified by the Biological Stain Commission, Dye content >=85 % | AKOS015894698 | 3,7-diamino-5lambda4-phenothiazin-5-ylium acetate | Thionin acetate salt, certified by the Biolog

Storage

Room temperature,Desiccated

Shipped In

Normal

Overview

Application：

Thionin acetate is a strongly staining metachromatic dye that is widely used for biological staining. Thionine can also be used in place of Schiff reagent in quantitative Feulgen staining of DNA. It can also be used to mediate electron transfer in microbial fuel cells. It is also employed as a general nuclear stain for chromatin and mucin; for staining Nissl granules by end-point buffering and for showing malignant cells in frozen biopsy sections; and as a stain for semi-thin sections of plant material for light microscopy.

Specifications

Synonyms

Thioninacetat | Thionin acetate (Thionine acetate) | Thionin acetate salt, certified by the Biological Stain Commission, Dye content >=85 % | AKOS015894698 | 3, 7-diamino-5lambda4-phenothiazin-5-ylium acetate | Thionin acetate salt, certified by the Biolog

Specifications & Purity

Dye content 85 %

Biochemical and Physiological Mechanisms

Thionine is a kind of heterochromatic CATIONIC dye. It is widely used in biological staining, especially in histology.

Storage

Room temperature, Desiccated

Shipped In

Normal

Names and Identifiers

• Pubchem Sid: 488192273
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/488192273
• Canonical Smiles: CC(=O)[O-].C1=CC2=C(C=C1N)SC3=CC(=[NH2+])C=CC3=N2
• IUPAC Name: (7-aminophenothiazin-3-ylidene)azanium;acetate
• InChIKey: JOIRQYHDJINFGA-UHFFFAOYSA-N
• INCHI: 1S/C12H9N3S.C2H4O2/c13-7-1-3-9-11(5-7)16-12-6-8(14)2-4-10(12)15-9;1-2(3)4/h1-6,13H,14H2;1H3,(H,3,4)
• Isomeric SMILES: CC(=O)[O-].C1=CC2=C(C=C1N)SC3=CC(=[NH2+])C=CC3=N2
• PubChem CID: 2724414
• Molecular Weight: 287.34

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Benzothiazines

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Benzothiazines
• Subclass: Not available
• Intermediate Tree Nodes: Not available
• Direct Parent: Benzothiazines
• Alternative Parents: Benzenoids Heteroaromatic compounds Monocarboxylic acids and derivatives Carboxylic acids Azacyclic compounds Primary amines Organopnictogen compounds Organic salts Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Not available
• Substituents: Benzothiazine - Benzenoid - Heteroaromatic compound - Carboxylic acid derivative - Carboxylic acid - Monocarboxylic acid or derivatives - Azacycle - Organic salt - Organic oxygen compound - Primary amine - Organooxygen compound - Organonitrogen compound - Organic nitrogen compound - Amine - Carbonyl group - Organopnictogen compound - Hydrocarbon derivative - Organic oxide - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as benzothiazines. These are organic compounds containing a benzene fused to a thiazine ring (a six-membered ring with four carbon atoms, one nitrogen atom and one sulfur atom).
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

Huh-7 (12904 Activities)

Explore this target

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Chemical and Physical Properties

• Solubility: Soluble in water (~2.5 mg/ml at 25°C), and HOAc: H2O (1:1) (1 mg/ml). Insoluble in ethanol.
• Molecular Weight: 287.340 g/mol
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 0
• Exact Mass: 287.073 Da
• Monoisotopic Mass: 287.073 Da
• Topological Polar Surface Area: 129.000 Ų
• Heavy Atom Count: 20
• Formal Charge: 0
• Complexity: 414.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 2

XLogP3

References

1. Yana Chen, Shuangling Wang, Jujie Ren, Haiyan Zhao, Min Cui, Na Li, Meng Li, Cong Zhang.  (2022)  Electrocatalysis of Copper Sulfide Nanoparticle-Engineered Covalent Organic Frameworks for Ratiometric Electrochemical Detection of Amyloid-β Oligomer.  ANALYTICAL CHEMISTRY,      [PMID:35920591] [10.1021/acs.analchem.2c01602]

2. Huawei Yang, Liangjiu Bai, Donglei Wei, Lixia Yang, Wenxiang Wang, Hou Chen, Yuzhong Niu, Zhongxin Xue.  (2018)  Ionic self-assembly of poly(ionic liquid)-polyoxometalate hybrids for selective adsorption of anionic dyes.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2018.10.100]

3. Xinming Wang, Yiming Zhong, Bei Qian, Shixing Huang, Qiang Long, Haonan Zhang, Qiang Zhao, Xiaofeng Ye.  (2025)  Self-assembled extracellular matrix-lipid nanoparticle composite for site-specific siRNA delivery to improve cardiac repair post-myocardial infarction.  Materials Today Bio,      [PMID:40893381] [10.1016/j.mtbio.2025.102205]