Unstained Protein MW Marker (10-235kDa) - for western blot, suitable for electrophoresis, 10-235kDa; 10/ 15/ 20/ 25/ 35/ 50/ 70/ 100/ 150/ 235kDa

Cat. No.: N1491669

GRADE & PURITY Suitable for electrophoresis, for Western blot, 10-235kDa; 10/ 15/ 20/ 25/ 35/ 50/ 70/ 100/ 150/ 235kDa

Synonyms

Unstained Protein Ladder

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

WB

Overview

This product consists of 10 recombinant proteins spanning a molecular weight range of 10–235 kDa. The marker provides sharp bands, accurate molecular weights, well-distributed size ranges, and excellent storage stability.
Protocol
Thaw the marker at room temperature for a few minutes before use. Gently mix to ensure homogeneity.
Load an appropriate volume into the gel wells:
Gel thickness 0.75–1.0 mm: Load 5 μL/well.
Gel thickness 1.5 mm: Load 10 μL/well.
Adjust the volume as needed based on experimental conditions.
After use, close the tube and store at 4°C or –20°C.
Note: For a 5 μL loading volume, each band contains approximately 0.5–1 μg of protein.
Precautions
This product can be heated/boiled (e.g., for denaturing gels).
In low-percentage gels, low-molecular-weight bands may co-migrate with the dye front and become unresolved.
To avoid repeated freeze-thaw cycles and contamination, aliquot and store at –20°C.
This product contains multiple chemical components. Do not ingest and avoid contact with eyes or skin. Wear a lab coat and disposable gloves during handling.
For research use only.

Specifications

Synonyms

Unstained Protein Ladder

Specifications & Purity

for western blot, suitable for electrophoresis, 10-235kDa; 10/ 15/ 20/ 25/ 35/ 50/ 70/ 100/ 150/ 235kDa

Stability And Storage

Store at 2-8℃ short term (3 months). Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

for Western blot, Suitable for electrophoresis

Images

SDS-PAGE band profile of Unstained Protein MW Marker

Unstained Protein MW Marker (10-235kDa) (N1491669) - Image is a 4-20% gels (SDS-PAGE) stained with Coomassie blue gel stain

The SDS-PAGE analysis of the Unstained Protein MW Marker (10-235 kDa) (N1491669) shows a clear and well-resolved band profile on both 4-20% Bis-Tris and 4-20% Tris-Glycine gels. The marker covers a broad molecular weight range from 10 kDa to 235 kDa, with distinct bands at key molecular weights, including: 10 kDa, 15 kDa, 20 kDa, 25 kDa, 35 kDa, 50 kDa, 70 kDa, 100 kDa, 150 kDa, 235 kDa. 

This profile demonstrates excellent separation and band sharpness, making it suitable for accurate molecular weight estimation of unknown proteins under standard SDS-PAGE conditions.