Xylenol Orange tetrasodium salt - Reagent grade , CAS No.3618-43-7

CAS: 3618-43-7 Cat. No.: X100265 Molecular Weight: 760.58 EC Number: 222-805-8

GRADE & PURITY Reagent Grade

Synonyms

3,3′-Bis[N,N-bis(carboxymethyl)aminomethyl]-o-cresolsulfonephthalein tetrasodium salt | Tetrasodium N,N'-[3H-2,1-benzoxathiol-3-ylidenebis[(6-hydroxy-5-methylphen-3,1-ylene)methylene]]bis[N-(carboxylatomethyl)aminoacetate] S,S-dioxide

Storage

Room temperature

Shipped In

Normal

Overview

Xylenol Orange tetrasodium salt is a fluorochrome acid dye with complexing and chelating properties that make it useful as an indicator in the spectrophotometric detection and assay of metal ions such as calcium, cadmium, bismuth, zinc, lead, and mercury. Its tetrasodium salt especially is used as an indicator in metal titrations. It changes color from yellow to red between pH 6.4 to 10.4. It has six potentially anionic substituents, one being sulfonate, and is anionic under staining conditions and during most laboratory applications.
Application
Xylenol Orange has been used as a vital stain to study calcification in bone development, injury, and repair.
It has been used in the fluorescent histochemical detection of alkaline phosphatase.
It has been used in FBX (ferrous benzoic xylenol orange) gel preparation to study the effects of MRI (magnetic resonance imaging) parameters on image quality.

Specifications

Synonyms

3, 3′-Bis[N, N-bis(carboxymethyl)aminomethyl]-o-cresolsulfonephthalein tetrasodium salt | Tetrasodium N, N'-[3H-2, 1-benzoxathiol-3-ylidenebis[(6-hydroxy-5-methylphen-3, 1-ylene)methylene]]bis[N-(carboxylatomethyl)aminoacetate] S, S-dioxide

Specifications & Purity

Reagent grade

Storage

Room temperature

Shipped In

Normal

Grade

Reagent Grade

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Refer to SDS for further information Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Names and Identifiers

• WGK Germany: 3
• Alternate CAS: 63721-83-5
• Molecular Weight: 760.58

Chemical and Physical Properties

• Solubility: Soluble in water.
• Sensitivity: Moisture sensitive.
• Melt Point(°C): 210°C

References

1. Gao Junkai, Liu Yu, Li Xuebin, Yang Mouyuan, Wang Jinbao, Chen Yan.  (2020)  A promising and cost-effective biochar adsorbent derived from jujube pit for the removal of Pb(II) from aqueous solution.  Scientific Reports,  10  (1): (1-13).  [PMID:32366969] [10.1038/s41598-020-64191-1]

2. Xiang-Bo Zeng, Fa-Wen Yin, Guan-Hua Zhao, Chao Guo, De-Yang Li, Hui-Lin Liu, Lei Qin, Fereidoon Shahidi, Da-Yong Zhou.  (2024)  Mechanism of color change in Antarctic krill oil during storage.  FOOD CHEMISTRY,      [PMID:38309082] [10.1016/j.foodchem.2024.138583]

3. Xiang-Bo Zeng, Xue-Chen Pei, De-Yang Li, Fa-Wen Yin, Hui-Lin Liu, Meng-Ling Jin, Jiang-Hua Zhang, Da-Yong Zhou.  (2024)  Mechanism of discoloration of Antarctic krill oil upon storage: A study based on model systems.  FOOD CHEMISTRY,      [PMID:39002334] [10.1016/j.foodchem.2024.140376]

4. Linna Han, Zixu Wang, Wanfang Feng, Qing Xia, Yucun Niu, Liyan Liu.  (2025)  Bromine (Br)-modified magnetic Zr metal organic framework for highly efficient adsorption and detection of sugar phosphates in biological samples.  ANALYTICA CHIMICA ACTA,      [PMID:41022510] [10.1016/j.aca.2025.344574]