α-2-3,6,8 sialidase (SpNanA), CAS No.9001-67-6

CAS: 9001-67-6 Cat. No.: S1524614 EC Number: 232-624-6

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥85%(SDS-PAGE), expressed in E.coli; ≥2000 U/mg protein; Protein concentration: See COA

Accession #

P62575

Protein Tag

C-His

Expression system

E. coli

Bioactivity

≥2000 U/mg protein

Overview

α-2,3,6,8-Sialidase (SpNanA), E.C 3.2.1.18, derived from Streptococcus pneumoniae. It is an exo-sialidase that catalyzes the hydrolysis of sialic acids linked via α2,3, α2,6 and α2,8 glycosidic bonds. This product is recombinantly expressed in Escherichia coli with high enzymatic activity.

Reaction

Protocol

Prepare the reaction mixture containing the substrate, 5 mM MgCl₂, and optionally a buffer salt (e.g., Tris‑HCl) to adjust the pH to 6.0. Add an appropriate amount of SpNanA according to the reaction requirements.

Mix well and incubate at 37 °C for 2–4 hours or overnight.

Note: For scaled‑up reactions, the incubation time or enzyme amount may need to be adjusted based on actual conditions. Low‑speed orbital shaking can be applied to ensure uniform mixing.

Precautions

For your safety and health, please wear a lab coat and disposable gloves during handling. This product is for research use only!

Specifications

Product Name

α-2-3,6,8 sialidase (SpNanA), CAS No.9001-67-6

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥85%(SDS-PAGE), expressed in E.coli; ≥2000 U/mg protein; Protein concentration: See COA

Bioactivity

≥2000 U/mg protein

Accession #

P62575

Predicted molecular weight

57 kDa

CAS

9001-67-6

Enzyme Commission Number

EC 3.2.1.18

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in E.coli; ≥2000 U/mg protein; Protein concentration: See COA

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

The amount of enzyme that catalyzes the hydrolysis of 1 nmol of Neu5Acα2,8Galβ1,4Glc to Galβ1,4Glc per minute at 37 °C and pH 6.0

References

1. Qian Wang, Zichao Peng, Bin Gui, Yongming Li, Lanjie Liao, Zuoyan Zhu, Yaping Wang, Libo He.  (2025)  Sialic acid serves as a functional receptor for grass carp reovirus.  PLoS Pathogens,  21  (9): (e1013481).  [PMID:40911632] [10.1371/journal.ppat.1013481]

2. Yifei Wang, Mengyi Zhang, Yimeng An, Lanshu Li, Hao Wu, Ziqi Cheng, Ling Pan, Chaoying Yang, Weijin Huang, Yansheng Geng, Chenyan Zhao.  (2025)  A Bioluminescent Imaging Mouse Model for Seasonal Influenza Virus Infection Based on a Pseudovirus System.  Viruses-Basel,  17  (5): (686).  [PMID:40431697] [10.3390/v17050686]