Different amino acids carry side chain groups of different chemical properties, some with basic side chains and some with acidic side chains. The proteins composed of them thus have different numbers of basic and acidic groups. These groups cause the protein to carry different net charges in solutions of different pH values. A protein with a net positive charge is defined as a basic protein (with an isoelectric point biased toward the acidic range). A protein with a net negative charge is defined as an acidic protein (with an isoelectric point biased toward the acidic range).
Acid Fast Green Staining Solution works based on the binding between acidic proteins and the positively charged acidic dye Fast Green. The most abundant acidic proteins in cells are mainly located in the cytoplasm and nucleoli. Therefore, most of the cytoplasm and nucleoli are stained green after staining. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
A1511551
| Component
| 2×50mL
| Storage
|
A1511551A
| Acid Fast Green A
| 50mL
| RT
|
A1511551B
| Acid Fast Green B
| 50mL
| RT |
Immediately before use, mix equal volumes of Solution A and Solution B to obtain 0.1% Acid Fast Green Staining Solution.
Materials Required (User-supplied):
1. Glass slides
2. 70% ethanol
3. Acid differentiation solution
4. Water bath
5. Microscope
Protocol (For Reference Only):
1. Place a drop of fresh blood on one end of a glass slide, spread into a smear, and air-dry at room temperature.
2. Immerse the smear in ethanol for fixation, then air-dry at room temperature.
3. Immerse the smear in acid differentiation solution and incubate in a water bath.
4. Wash thoroughly with running water, and absorb residual moisture with filter paper.
5. Immerse the smear in Acid Fast Green staining solution for staining.
6. Rinse with running water and air-dry at room temperature.
7. Examine microscopically directly, or add 1 drop of neutral balsam and cover with a coverslip for mounting and observation.
Staining Results:
Cytoplasm, nucleoli: Green
Most regions of the cell nucleus: Unstained
Precautions:
1. Blood smears or bone marrow smears should be of uniform thickness to avoid affecting the staining result.
2. Fresh whole blood or EDTA anticoagulated blood is required for blood cell smear staining.
3. After incubation in acid differentiation solution, thorough rinsing is necessary; otherwise, it will interfere with Fast Green staining.
4. If overstained, appropriate decolorization can be performed with methanol or ethanol; restaining is not recommended.
5. pH value has a certain influence on staining. Glass slides should be clean and free from acid or alkali contamination to avoid affecting the staining result.
6. For your safety and health, please wear a lab coat and disposable gloves during operation.
7. This product is for research use only, strictly prohibited for other purposes.
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