DFHBI 1T - ≥98%(HPLC) , CAS No.1539318-36-9

CAS: 1539318-36-9 Cat. No.: D288318 Molecular Weight: 320.21

GRADE & PURITY ≥98%(HPLC)

Synonyms

(5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Product description：

DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells.

Specifications

Synonyms

(5Z)-5-[(3, 5-Difluoro-4-hydroxyphenyl)methylene]-3, 5-dihydro-2-methyl-3-(2, 2, 2-trifluoroethyl)-4H-imidazol-4-one

Specifications & Purity

≥98%(HPLC)

Biochemical and Physiological Mechanisms

Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spin

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Purity

≥98%(HPLC)

Names and Identifiers

• Pubchem Sid: 488202595
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/488202595
• Canonical Smiles: CC1=NC(=CC2=CC(=C(C(=C2)F)O)F)C(=O)N1CC(F)(F)F
• IUPAC Name: (5Z)-5-[(3,5-difluoro-4-hydroxyphenyl)methylidene]-2-methyl-3-(2,2,2-trifluoroethyl)imidazol-4-one
• InChIKey: AWYCLBWNRONMQC-WMZJFQQLSA-N
• INCHI: 1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4-
• Isomeric SMILES: CC1=N/C(=C\C2=CC(=C(C(=C2)F)O)F)/C(=O)N1CC(F)(F)F
• Molecular Weight: 320.21
• Reaxy-Rn: 54958173
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=54958173&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives
• Direct Parent: Alpha amino acids and derivatives
• Alternative Parents: O-fluorophenols Fluorobenzenes Imidazolinones Aryl fluorides Propargyl-type 1,3-dipolar organic compounds Carboximidamides Carboxamidines Azacyclic compounds Organofluorides Organic oxides Hydrocarbon derivatives Carbonyl compounds Alkyl fluorides
• Molecular Framework: Aromatic heteromonocyclic compounds
• Substituents: Alpha-amino acid or derivatives - 2-halophenol - 2-fluorophenol - Fluorobenzene - Halobenzene - Phenol - Aryl fluoride - Aryl halide - Monocyclic benzene moiety - Imidazolinone - Benzenoid - 2-imidazoline - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Carboximidamide - Organoheterocyclic compound - Azacycle - Amidine - Carboxylic acid amidine - Organooxygen compound - Organic oxide - Organic oxygen compound - Hydrocarbon derivative - Carbonyl group - Organic nitrogen compound - Alkyl halide - Alkyl fluoride - Organohalogen compound - Organofluoride - Organonitrogen compound - Aromatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as alpha amino acids and derivatives. These are amino acids in which the amino group is attached to the carbon atom immediately adjacent to the carboxylate group (alpha carbon), or a derivative thereof.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Solvent:DMSO, Max Conc. mg/mL: 32.02, Max Conc. mM: 100; Solvent:ethanol, Max Conc. mg/mL: 16.01, Max Conc. mM: 50
• Sensitivity: Light sensitive
• Molecular Weight: 320.210 g/mol
• XLogP3: 2.500
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 8
• Rotatable Bond Count: 2
• Exact Mass: 320.058 Da
• Monoisotopic Mass: 320.058 Da
• Topological Polar Surface Area: 52.900 Ų
• Heavy Atom Count: 22
• Formal Charge: 0
• Complexity: 505.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 1
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 1
• Covalently-Bonded Unit Count: 1

References

1. Yuan Chen, Hullahalli Karthik, Huang Hao, Zhao Siqi, Wang Wenqing, Tian Xingyu, Li Xin, Xia Linya, Wang Yuchang, Pan Fei, Liang Ying, Xie Yurui, Li Yue, Fan Hongjie, Waldor Matthew K., Ma Zhe.  (2025)  Zoonotic Streptococcus imports glucose to inhibit stringent response and promote growth during meningitis.  Nature Microbiology,      [PMID:41398396] [10.1038/s41564-025-02194-2]