10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 8 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
Indirubin (NSC 105327) is a potent cyclin-dependent kinases and GSK-3β inhibitor with IC50 of about 5 μM and 0.6 μM.
In vitro
Indirubin is the active ingredient of Danggui Longhui Wan, a mixture of plants that is used in traditional Chinese medicine to treat chronic diseases. Indirubin inhibits CDKs activity with IC50 of 2.2 - 10 μM resulting in cell cycle arrest in the G2/M phase. Indirubin also inhibits GSK-3β with an IC50 of 0.6 μM, attenuating CDK5- and GSK-3β-mediated tau phosphorylation, a process over-active in Alzheimer disease states. It also suppresses tumor necrosis factor (TNF)-induced NF-κB activation in a dose- and time-dependent manner. Indirubin also suppresses the NF-κB activation induced by various inflammatory agents and carcinogens. Indirubin blocks the phosphorylation and degradation of IκBα through the inhibition of activation of IκBα kinase and phosphorylation and nuclear translocation of p65.
In vivo
Cell Data
cell lines：Murine ID8 ovarian carcinoma cells
Concentrations：
Incubation Time：
Powder Purity:≥97%
| Isomeric SMILES | C1=CC=C2C(=C1)C(=C(N2)O)C3=NC4=CC=CC=C4C3=O |
|---|---|
| Molecular Weight | 262.26 |
| Reaxy-Rn | 30107462 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=30107462&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Solubility | Solubility (25°C) In vitro DMSO: 45 mg/mL (196.08 mM); Water: 45 mg/mL (196.08 mM); Ethanol: 45 mg/mL (196.08 mM); |
|---|---|
| Melt Point(°C) | 350°C(lit.) |
| 1. Mingyi Yao, Jian Liu, Narengaowa, Yonggu Li, Feng Zhao. (2023) In situ detection of natural dyes in archaeological textiles by SERS substrates immobilized on the fiber. MICROCHEMICAL JOURNAL, [PMID:] [10.1016/j.microc.2023.108939] |
| 2. Sun Bing-Yao, Sui Hua-Lu, Liu Zi-Wei, Tao Xin-Yi, Gao Bei, Zhao Ming, Ma Yu-Shu, Zhao Jian, Liu Min, Wang Feng-Qing, Wei Dong-Zhi. (2022) Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin. Bioresources and Bioprocessing, 9 (1): (1-16). [PMID:38647553] [10.1186/s40643-022-00559-7] |
| 3. Huifang Yin, Hongping Chen, Meng Yan, Zhikun Li, Rongdi Yang, Yanjiao Li, Yanfang Wang, Jianyi Guan, Huili Mao, Yan Wang, Yuyang Zhang. (2021) Efficient Bioproduction of Indigo and Indirubin by Optimizing a Novel Terpenoid Cyclase XiaI in Escherichia coli. ACS Omega, [PMID:34396002] [10.1021/acsomega.1c02679] |
| 4. Wu Xunxun, Chen Xiaofei, Jia Dan, Cao Yan, Gao Shouhong, Guo Zhiying, Zerbe Philipp, Chai Yifeng, Diao Yong, Zhang Lei. (2016) Characterization of anti-leukemia components from Indigo naturalis using comprehensive two-dimensional K562/cell membrane chromatography and in silico target identification. Scientific Reports, 6 (1): (1-13). [PMID:27150638] [10.1038/srep25491] |
| 5. Ling Wang, Xianglu Li, Xinyu Liu, Kang Lu, Na Chen, Peipei Li, Xiao Lv, Xin Wang. (2015) Enhancing effects of indirubin on the arsenic disulfide-induced apoptosis of human diffuse large B‑cell lymphoma cells. Oncology Letters, 9 (4): (1940-1946). [PMID:25789073] [10.3892/ol.2015.2941] |
| 6. Yancen Li, Chengyu Wan, Fan Li, Guang Xin, Tao Wang, Qilong Zhou, Tingyu Wen, Shiyi Li, Xiaoting Chen, Wen Huang. (2025) Indirubin attenuates sepsis by targeting the EGFR/SRC/PI3K and NF-κB/MAPK signaling pathways in macrophages. Frontiers in Pharmacology, [PMID:40144662] [10.3389/fphar.2025.1542061] |
| 7. Changwei Zhang, Hua Yuan, Hong Shen, Chuan Li, Jianzhong Ye, Huaxing Zhang, Chengzhang Wang. (2024) Physicochemical properties and antibacterial activity evaluation of new indigo extract from Baphicacanthus cusia (Nees) Bremek prepared by chemical conversion method. Arabian Journal of Chemistry, [PMID:] [10.1016/j.arabjc.2024.105801] |
| 8. Ma Ke-Yu, Hou Bao-Long, Fan An-Qi, Huang An-Li, Li Bo-Rui, Liang Yan-Ni, Wang Zheng. (2026) The mechanism of indigo naturalis and its active ingredients against ulcerative colitis. Scientific Reports, [PMID:41535567] [10.1038/s41598-026-35413-9] |
