Methyl Green Staining Solution (0.1%) - BioReagent,Biological Stain,for microscopy,0.1%

Cat. No.: M1508539

GRADE & PURITY BioReagent, Biological Stain, for Microscopy, 0.1%

Synonyms

Methyl Green Stain

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining, IF/ICC, IHC

Overview

  Methyl Green Staining Solution (0.1%) is a commonly used staining solution in tissue or cell staining that colors the nucleus green. Methyl Green binds to DNA in the nucleus, resulting in nuclear staining. This staining solution can be used in combination with immunofluorescence staining or immunohistochemical staining. On one hand, immunofluorescence staining or staining with other dyes can be performed after staining with this Methyl Green solution; on the other hand, Methyl Green can also be used as a counterstain after immunohistochemical staining.

  10 mL of staining solution can stain approximately 20 samples. This reagent is intended for research use only and is not suitable for clinical diagnosis or other purposes.

Instructions for Use

1. Sample Processing

1.1 For Paraffin Sections

• Deparaffinize in xylene for 5–10 minutes.

• Replace with fresh xylene and deparaffinize for another 5–10 minutes.

• Absolute ethanol for 5 minutes.

• 90% ethanol for 2 minutes.

• 70% ethanol for 2 minutes.

• Distilled water for 2 minutes.

1.2 For Frozen Sections

• Distilled water for 2 minutes.

1.3 For Cultured Cells

• Fix with 4% paraformaldehyde for at least 10 minutes.

• Wash with distilled water for 2 minutes.

• Replace with fresh distilled water and wash for another 2 minutes.

2. Methyl Green Staining

• For the processed samples above, stain with Methyl Green Staining Solution (0.1%) for 5–10 minutes.

• Rinse twice with distilled water. The samples will appear blue at this stage.

• Treat with 95% ethanol for 5 seconds.

• Wash twice with 70% ethanol. Observe directly or proceed with the following tissue section steps.

3. Staining of Tissue Sections

• Dehydrate with 95% ethanol for 2 minutes.

• Replace with fresh 95% ethanol and dehydrate for another 2 minutes.

• Clear with xylene for 5 minutes.

• Clear again with xylene for 5 minutes.

• Mount with neutral balsam or other mounting medium.

• Observe under a microscope: nuclei appear green or bluish‑green.

4. Fluorescence Staining

• If performing immunofluorescence staining, after staining with Methyl Green Staining Solution, wash twice with 70% ethanol, 2 minutes each time.

• Dehydrate with 95% ethanol for 2 minutes.

• Soak in PBS, physiological saline, TBS, or other solutions used for immunostaining or fluorescent dye staining for 5 minutes.

• Proceed with immunofluorescence staining or other fluorescent dye staining.

Staining Results

  Nuclei appear green or bluish‑green.

Notes

1. Users need to prepare 4% paraformaldehyde, distilled water, graded ethanol series, xylene, etc., themselves.

2. When using this kit for the first time, it is recommended to perform a preliminary experiment with 1–2 samples.

3. The staining time with Methyl Green can be adjusted according to staining results and requirements.

4. Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.

5. This product is restricted to scientific research by professionals and must not be used for clinical diagnosis or treatment, nor for food or pharmaceuticals. It should not be stored in ordinary residential areas.

6. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

Synonyms

Methyl Green Stain

Specifications & Purity

BioReagent, Biological Stain, for microscopy, 0.1%

Stability And Storage

Store at room temperature long term (12 months). Store in the dark.

Storage

Protected from light, Room temperature

Shipped In

Normal

Grade

Biological Stain, BioReagent, for Microscopy

Chemical and Physical Properties

• Sensitivity: Light-sensitive