Mouse Anti-Human IgG (PerCP-Cy5.5) - Secondary antibody, specific to Human IgG

Cat. No.: Ab226757
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5mg/mL
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG1
Clone Number
1H8
Conjugation
PerCP-Cy5.5
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab226757-10μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$19.90
100μg
Ab226757-100μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$99.90
500μg
Ab226757-500μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$299.90
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Validated, 0.5mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse Anti-Human IgG Antibody (mAb), Secondary antibody, could be used for Flow Cytometry.

Specifications

Product Name
Mouse Anti-Human IgG (PerCP-Cy5.5) - Secondary antibody, specific to Human IgG
Synonyms
Mouse Anti-Human IgG Antibody | Mouse Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human IgG Antibody | Human IgG Antibody | FLJ39988 antibodyFLJ40036 antibody | FLJ40253 antibody | FLJ40587 antibody | FLJ40789 antibody | FLJ40834 antibody | G
Specifications & Purity
ExactAb™, Validated, 0.5mg/mL
Host species
Mouse
Specificity
Human IgG
Immunogen
Human IgG
Conjugation
PerCP-Cy5.5
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
690nm
Ex/Em(nm)
Ex:482nm;Em:690nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Octylic acid-ammonium sulfate precipitation
Concentration
0.5mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

Mouse Anti-Human IgG (PerCP-Cy5.5) (Ab226757) - Flow Cytometry
Two-color flow cytometric analysis of IgG expression on viable Human peripheral blood lymphocytes. Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (AF405) (Ab223516) and either Mouse IgG (PerCP-Cy5.5) (Ab190081; Left panel) or Mouse Anti-Human IgG (PerCP-Cy5.5) (Ab226757; Right panel) at 0.3μg/test.

Mouse Anti-Human IgG (PerCP-Cy5.5) (Ab226757) - Flow Cytometry
Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902). Mouse Anti-Human IgG (PerCP-Cy5.5) (Ab226757) at a dilution of 1/500 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

1/50 - 1/1000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
Reviews

Customer Reviews

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Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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