GRADE & PURITYSterile-filtered?Sterile-filtered — passed through a 0.2µm filter to remove microorganisms. Use as a ready aseptic solution for culture and sensitive applications.for Cell culture?Cell-culture grade — low endotoxin and contaminants to support viable cell growth. Use in mammalian/other cell culture media and supplements.With L-glutamine and sodium bicarbonate, liquid
sterile-filtered, for cell culture, With L-glutamine and sodium bicarbonate, liquid for Cell culture,Sterile-filtered for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.
Application:
Nutrient Mixture F-12 Ham has been used:
to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
to culture LAN-1 cells
to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells
with Dulbecco’s modified Eagle’s medium (DMEM) media in a 1:1 ratio to culture oral cavity squamous cell carcinoma (OSCC) cell lines.
Specifications
Specifications & Purity
sterile-filtered, for cell culture, With L-glutamine and sodium bicarbonate, liquid
Storage
Store at 2-8°C
Shipped In
Wet ice
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
for Cell culture, Sterile-filtered
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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