Poly-D-lysine hydrobromide - γ-irradiated, BioReagent, for cell culture, average mol wt 30,000-70,000, lyophilized powder , CAS No.27964-99-4

CAS: 27964-99-4 Cat. No.: P755746 EC Number: 959-037-4 PubChem CID: 87493163

GRADE & PURITY BioReagent, for Cell culture, γ-irradiated, average mol wt 30,000-70,000, lyophilized powder

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Poly-D-Lysine Hydrobromide (PDL), a positively charged polymer consisting of lysine residues, where each lysine unit is associated with a hydrobromide molecule. This hydrobromide molecule gives the poly-D-lysine a unique characteristic, enabling it to form crystals and making it easily soluble in water-based solutions.

Application:

Poly d lysine hydrobromide coating has been used:

• In coating tissue culture plate wells and coverslips for immunofluorescence microscopy of primary neuronal culture to study neurological disorders associated with Zika virus infection.
• To coat the coverslips for enhancing the attachment and proliferation of the myenteric cells isolated from the rat intestines.
• In preparing surface of coverslips for cell attachment for studying the myenteric plexus of rat’s small intestine.

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm². Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Specifications

Specifications & Purity

γ-irradiated, BioReagent, for cell culture, average mol wt 30, 000-70, 000, lyophilized powder

Biochemical and Physiological Mechanisms

Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for Cell culture, γ-irradiated

Names and Identifiers

• Canonical Smiles: C(CCN)CC(C(=O)O)N.Br
• IUPAC Name: (2R)-2,6-diaminohexanoic acid;hydrobromide
• InChIKey: MEXAGTSTSPYCEP-NUBCRITNSA-N
• INCHI: 1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H/t5-;/m1./s1
• Isomeric SMILES: C(CCN)C[C@H](C(=O)O)N.Br
• PubChem CID: 87493163

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives
            • Level7 Alpha amino acids
              • Level8 D-alpha-amino acids

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives - Alpha amino acids and derivatives - Alpha amino acids
• Direct Parent: D-alpha-amino acids
• Alternative Parents: Medium-chain fatty acids Amino fatty acids Amino acids Monocarboxylic acids and derivatives Carboxylic acids Organic oxides Monoalkylamines Hydrocarbon derivatives Hydrobromides Carbonyl compounds
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: D-alpha-amino acid - Medium-chain fatty acid - Amino fatty acid - Fatty acid - Fatty acyl - Amino acid - Carboxylic acid - Monocarboxylic acid or derivatives - Amine - Hydrobromide - Hydrocarbon derivative - Primary amine - Organooxygen compound - Organonitrogen compound - Organic oxide - Primary aliphatic amine - Organic oxygen compound - Carbonyl group - Organic nitrogen compound - Aliphatic acyclic compound
• Description: This compound belongs to the class of organic compounds known as d-alpha-amino acids. These are alpha amino acids which have the D-configuration of the alpha-carbon atom.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: H 2 O: soluble 50 mg/mL, clear, colorless
• Flash Point(°F): Not applicable
• Flash Point(°C): Not applicable

References

1. Hai-Mu Ye, Shu-Fang Yao.  (2017)  Supernucleating Role of Poly(ω-pentadecalactone) during the Crystallization of Poly(ε-caprolactone) Composites.  INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,      [PMID:] [10.1021/acs.iecr.7b03322]