Puromycin Hydrochloride Solution (1 mg/mL) - BioReagent,for cell culture,1 mg/ml , CAS No.58-58-2

CAS: 58-58-2 Cat. No.: P1509572 Molecular Weight: 544.44 Beilstein Registry Number: 3853613 EC Number: 200-387-8

GRADE & PURITY BioReagent, for Cell culture, 1 mg/ml

Synonyms

Puromycin Hydrochloride Solution

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Overview

  Puromycin (PM) is a protein synthesis inhibitor with a CAS number of 53-79-2. It has a structure similar to the terminal end of tRNA molecules, which enables it to bind to amino acids, replace aminoacylated tRNA to bind to the A-site of ribosomes, and be incorporated into the growing peptide chain. Although puromycin can bind to the A-site, it cannot participate in any subsequent reactions, thus terminating protein synthesis and releasing immature polypeptides containing puromycin at the C-terminus. Puromycin dihydrochloride is an aminonucleoside antibiotic produced by the fermentation of Streptomyces alboniger. As a stable hydrochloride form of puromycin, it is often referred to as puromycin hydrochloride, with a CAS number of 58-58-2.

  Puromycin Hydrochloride Solution (1 mg/mL) is formulated with high-purity raw materials and methanol. If necessary, it should be filtered through an organic filter membrane before being used for cell culture. Its molar concentration is 1.84 mmol/L, and the general working concentration ranges from 10 to 100 μmol/L. This product is for research use only (RUO) and is not intended for clinical diagnosis or any other applications.

Operating Procedures (For Reference Only)

1. Operate in accordance with specific experimental requirements.

2. Taking a working concentration of 100 μmol/L as an example, add 544 μL of  Puromycin Hydrochloride Solution (1 mg/mL) to every 10 mL of culture medium.

Precautions

1. Ensure sterile operation to avoid contamination. Minimize repeated freeze-thaw cycles to prevent reagent inactivation.

2. For your safety and health, wear a lab coat and disposable gloves during operation.

Specifications

Synonyms

Puromycin Hydrochloride Solution

Specifications & Purity

BioReagent, for cell culture, 1 mg/ml

Stability And Storage

Store at -20℃ long term (12 months). Store in the dark.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for Cell culture

Names and Identifiers

• Isomeric SMILES: CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl
• WGK Germany: 3
• RTECS: AU7355000
• Alternate CAS: 53-79-2
• Molecular Weight: 544.44
• Beilstein: 3853613
• Reaxy-Rn: 39769009
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=39769009&ln=

Chemical and Physical Properties

• Sensitivity: Light-sensitive
• Melt Point(°C): 174°C(lit.)

References

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