Rat IgG1-kappa (Biotin) - Isotype Control Antibody

Cat. No.: Ab225919
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Isotype
Rat IgG1
Clone Number
HRPN
Conjugation
Biotin
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab225919-10μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$19.90
25μg
Ab225919-25μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$34.90
100μg
Ab225919-100μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$81.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

Recombinant, ExactAb™, Validated, 0.5 mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Rat IgG1 isotype control-InVivo monoclonal antibody (Clone: HRPN) reacts with horseradish peroxidase (HRP). Because HRP is not expressed by mammals this antibody is ideal for use as an isotype-matched control for rat IgG1 antibodies in most in vivo and in vitro applications. This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension, this can be used at the same concentration as the experimental antibody.

Specifications

Product Name
Rat IgG1-kappa (Biotin) - Isotype Control Antibody
Synonyms
Rat IgG1 | Rat IgG1 Isotype Control | Rat IgG1 antibody | Rat IgG1 Isotype Control antibody
Specifications & Purity
Recombinant, ExactAb™, Validated, 0.5 mg/mL
Host species
Rat
Conjugation
Biotin
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

Rat IgG1-kappa (Biotin) (Ab225919) - Flow Cytometry
C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were stained with Rat IgG1-kappa (Biotin) (Ab225919) (right panel). Then incubated with Streptavidin protein (FITC) (rp155876) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Unlabelled control, cells without incubation with primary antibody.

Rat IgG1-kappa (Biotin) (Ab225919) - Flow Cytometry
C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Rat IgG1-kappa (Biotin) (Ab225919) (blue). Then incubated with Streptavidin protein (FITC) (rp155876) at 0.1μg/test for 30 minutes at 4°C in the dark. Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

Use at an assay dependent concentration.
Each lot of this antibody is quality control tested by flow cytometric analysis as negative control. Use at concentrations comparable to those of the speci

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ25F0926883Certificate of AnalysisJul 14, 2026 Ab225919
ZJ25F0926884Certificate of AnalysisJul 14, 2026 Ab225919
ZJ25F0926885Certificate of AnalysisJul 14, 2026 Ab225919
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Recombinant grade guide → View Validated grade guide → View ExactAb™ grade guide →

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