Recombinant CD14 Antibody (Pacific Blue) - Primary antibody, specific to Human CD14

Cat. No.: Ab223671
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 5 μL/test
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human
Isotype
Mouse IgG2a
Clone Number
P25A37
Conjugation
Pacific Blue
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10T
Ab223671-10T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$59.90
25T
Ab223671-25T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$109.90
100T
Ab223671-100T
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$249.90
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™, Validated, Recombinant, 5 μL/test ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Recombinant CD14 Antibody, clone P25A37, could be used for Flow Cytometry.

Specifications

Product Name
Recombinant CD14 Antibody (Pacific Blue) - Primary antibody, specific to Human CD14
Synonyms
CD14 antigen | CD14 molecule | CD14 | monocyte differentiation antigen CD14 | Myeloid cell-specific leucine-rich glycoprotein | CD 14 antibody | CD_antigen=CD14 antibody | CD14 antibody | CD14 antigen antibody | CD14 molecule antibody | CD14_HUMAN antibod
Specifications & Purity
ExactAb™, Validated, Recombinant, 5 μL/test
Biochemical and Physiological Mechanisms
Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP a
Host species
Mouse
Specificity
Human CD14
Immunogen
Full-length human CD14 protein
Positive Control
Flow Cytometry: Human peripheral blood monocytes
Conjugation
Pacific Blue
Excitation (nm)
Violet Laser(405nm)
Emission(nm)
455nm
Ex/Em(nm)
Ex:410nm;Em:455nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
5 μL/test
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images

Recombinant CD14 Antibody (Pacific Blue) (Ab223671) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD14 Antibody (Pacific Blue) (Ab223671) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (Pacific Blue) (Ab216764).

Recombinant CD14 Antibody (Pacific Blue) (Ab223671) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD14 Antibody (Pacific Blue) (Ab223671) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (Pacific Blue) (Ab216764).

Application
ApplicationDilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
CD14 Tbio Monocyte differentiation antigen CD14 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ25F1028187Certificate of AnalysisJul 07, 2026 Ab223671
ZJ25F1028188Certificate of AnalysisJul 07, 2026 Ab223671
ZJ25F1028189Certificate of AnalysisJul 07, 2026 Ab223671
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