Recombinant CD25 Antibody (AF405) - Primary antibody, specific to Mouse CD25

Cat. No.: Ab230865
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG1
Clone Number
P18B24
Conjugation
AF405
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab230865-10μg
8-12 wks(?) Production requires sourcing of materials. We appreciate your patience and understanding.
$49.90
25μg
Ab230865-25μg
8-12 wks(?) Production requires sourcing of materials. We appreciate your patience and understanding.
$90.90
100μg
Ab230865-100μg
8-12 wks(?) Production requires sourcing of materials. We appreciate your patience and understanding.
$209.90
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Why this grade

Recombinant, ExactAb™, Validated, 0.5mg/mL ExactAb™,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Recombinant CD25 Antibody, clone P18B24, could be used for Flow Cytometry.

Specifications

Product Name
Recombinant CD25 Antibody (AF405) - Primary antibody, specific to Mouse CD25
Synonyms
Interleukin 2 receptor alpha chain antibody | CD25 antibody | CD25 antigen antibody | IDDM10 antibody | IL 2 receptor alpha subunit antibody | IL-2 receptor subunit alpha antibody | IL-2-RA antibody | IL-2R subunit alpha antibody | IL2 RA antibody | IL2 R
Specifications & Purity
Recombinant, ExactAb™, Validated, 0.5mg/mL
Biochemical and Physiological Mechanisms
Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Host species
Rat
Specificity
Mouse CD25
Immunogen
IL-2-dependent cytolytic mouse T-cell clone B6.1
Positive Control
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation
AF405
Excitation (nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
lambda
Purification Method
Protein A purified
Concentration
0.5mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application
ApplicationDilution info
Flow Cytometry

≤1.0μg/ test

For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
IL2RA Tclin Interleukin-2 receptor subunit alpha (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
Reviews

Customer Reviews

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