GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane b
Host species
Rat
Specificity
CD3
Immunogen
γδTCR-positive T-T hybridoma D1
Positive Control
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation
PerCP-Cy5.5
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
690nm
Ex/Em(nm)
Ex:482nm;Em:690nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Recombinant CD3 Antibody (PerCP-Cy5.5) (Ab230533) - Flow Cytometry Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD3 Antibody (PerCP-Cy5.5) (Ab230533) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (PerCP-Cy5.5) (Ab190075).
Recombinant CD3 Antibody (PerCP-Cy5.5) (Ab230533) - Flow Cytometry Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were preincubated with Mouse FcR Blocking Reagent (Ab219990). The cells were then stained with Recombinant CD3 Antibody (PerCP-Cy5.5) (Ab230533) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (PerCP-Cy5.5) (Ab190075). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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