GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.≥95%(SDS-PAGE)See COA
Carrier Free,Recombinant,ExactAb™,Azide Free,Validated,High Performance,≥95%(SDS-PAGE),See COA Azide Free,Carrier Free,ExactAb™,High Performance,Recombinant,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Recombinant F4/80 Antibody, clone P42A22, could be used for Flow Cytometry and so on.
Specifications
Product Name
Recombinant F4/80 Antibody - Primary antibody, specific to F4/80
Synonyms
ADGRE1 antibody | Adhesion G protein coupled receptor E1 antibody | Adhesion G protein-coupled receptor E1 antibody | AGRE1_HUMAN antibody | Cell surface glycoprotein EMR1 antibody | Cell surface glycoprotein F4/80 antibody | DD7A5 7 antibody | Egf like m
Specifications & Purity
Carrier Free, Recombinant, ExactAb™, Azide Free, Validated, High Performance, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Host species
Rat
Specificity
F4/80
Positive Control
Flow: C57BL/6 Mouse bone marrow
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Recombinant, Validated
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Two-color flow cytometric analysis of F4/80 expression on mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with CD11b Rat mAb (APC) (Ab178881) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or biotin-labeled Recombinant F4/80 Antibody (Ab222907; Right panel) at 1 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD11b versus F4/80 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.
Recombinant F4/80 Antibody (Ab222907) - SEC
The purity of Recombinant F4/80 Antibody (Ab222907) is more than 95% verified by HPLC.
Application
Application
Dilution info
Flow Cytometry
≤0.5 µg per million cells in 100 µl volume
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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