Recombinant Firefly Luciferase, CAS No.61970-00-1

CAS: 61970-00-1 Cat. No.: L465055 EC Number: 263-359-4
AVAILABLE TO ORDER
GRADE & PURITY Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Endotoxin tested ? Endotoxin-tested — each lot assayed and labeled with its endotoxin level. Use when you need a documented endotoxin value for risk assessment. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. ≥95% ≥2.0 × 10^11 RLU/mg protein; expressed in E.coli
Accession #
P08659
Expression system
E.coli & Pichia pastoris
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
≥2.0 × 10^11 RLU/mg protein
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
1mg
L465055-1mg
8-12 wks(?) Production requires sourcing of materials. We appreciate your patience and understanding.
$139.90
5mg
L465055-5mg
3
$599.90
Enter a quantity for the sizes you want to add.
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Why this grade

Recombinant, endotoxin tested, EnzymoPure™, sterile, ≥95%, ≥2.0 × 10^11 RLU/mg protein; expressed in E.coli Endotoxin tested,Recombinant,Sterile,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 2 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application:

Reporter Gene Assay; Bioluminescence; ATP Assay; Cell Viability Assay

Specifications

Product Name
Recombinant Firefly Luciferase, CAS No.61970-00-1
Synonyms
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) | firefly luciferase | luciferase (firefly luciferin) | Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing) | firefly luciferin luciferase | Photinus pyralis luciferase | Photinus-lu
Grade
Endotoxin tested, Recombinant, Sterile, EnzymoPure™
Specifications & Purity
Recombinant, endotoxin tested, EnzymoPure™, sterile, ≥95%, ≥2.0 × 10^11 RLU/mg protein; expressed in E.coli
Biochemical and Physiological Mechanisms
Produces green light with a wavelength of 562 nm.
Bioactivity
≥2.0 × 10^11 RLU/mg protein
Endotoxin Concentration
<1.0 EU/μg
Accession #
Predicted molecular weight
62 kDa
CAS
61970-00-1
Enzyme Commission Number
1.13.12.7
Molecule Type
Enzyme
Storage and Shipping
Concentration
≥2.0 × 10^11 RLU/mg protein; expressed in E.coli
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Unit definition
One relative light unit (RLU) is defined as the amount of enzyme which emits 1 count of light for 20 s at 30°C and pH 7.8.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot NumberCertificate TypeDateItem
J2522549Certificate of AnalysisOct 13, 2025 L465055
J2522551Certificate of AnalysisOct 13, 2025 L465055
H2223460Certificate of AnalysisOct 10, 2025 L465055
H2223461Certificate of AnalysisJul 06, 2022 L465055
Documents & Articles
Citations of This Product
References
1. Wang Zhengwei, Lin Jiani, Tan Guisen, Pan Tingzheng, Zhang Hongtian, Liu Jiangang.  (2025)  miR-1270 suppresses glioblastoma development by transcriptional inhibition of nuclear factor IX.  MOLECULAR BIOLOGY REPORTS,  52  (1): (1-10).  [PMID:40815328] [10.1007/s11033-025-10915-3]
2. Weiwei Liu, Qiuyue Ou, Meichang Feng, Yue Li, Linghan Huang, Xuan Peng, Xiaoqiang Zhong, Guoqiang Huang, Hengbo Wang, Jinlong Guo.  (2025)  Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation.  PLANT CELL AND ENVIRONMENT,      [PMID:40947544] [10.1111/pce.70180]
Solution Calculators
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