Recombinant Human AFP Protein

Cat. No.: rp169544

GRADE & PURITY Animal Free, Carrier Free, Bioactive, ActiBioPure™, His Tag, PBS Only, ≥90%(SDS-PAGE)

Expression System

HEK293

Accession #

P02771

Protein Tag

C-His

Expression system

HEK293

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Immobilized Recombinant Human AFP Protein (rp169544) at 1.0 μg/mL can bind AFP Mouse mAb (Ab007318) with the EC50 of 41.5 ng/mL.​

Overview

Purity: ≥90%, by SDS-PAGE visualized with Coomassie® Blue Staining.
Description：
Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).

Specifications

Product Name

Recombinant Human AFP Protein

Synonyms

Alpha-1-fetoprotein | Alpha-fetoglobulin | Alpha-fetoprotein | Hereditary persistence of alpha fetoprotein | Alpha fetoprotein precursor | alpha-fetoprotein, Hereditary persistence of, included | AFP | HPAFP

Grade

ActiBioPure™, Animal Free, Bioactive, Carrier Free, His Tag, PBS Only

Specifications & Purity

Animal Free, Carrier Free, Bioactive, ActiBioPure™, His Tag, PBS Only, ≥90%(SDS-PAGE)

Bioactivity

Immobilized Recombinant Human AFP Protein (rp169544) at 1.0 μg/mL can bind AFP Mouse mAb (Ab007318) with the EC50 of 41.5 ng/mL.​

Endotoxin Concentration

<1.0 EU/μg

Expression System

HEK293

Amino Acids

1-609 aa

Sequence

MKWVESIFLIFLLNFTESRTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGVHHHHHH

Protein Tag

C-His

Accession #

P02771

Predicted molecular weight

67.3 kDa

SDS-PAGE

67.5 kDa, under reducing conditions; 57.0 kDa, under non-reducing conditions

Molecule Type

Protein

Storage and Shipping

Shape

Lyophilized

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for 1 year. Avoid freeze / thaw cycle.

Images

SDS-PAGE: Recombinant Human AFP Protein (rp169544)

Recombinant Human AFP Protein (rp169544) - SDS-PAGE
3 μg/lane of Recombinant Human AFP Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 67.5 kDa under reducing conditions and 57.0 kDa under non-reducing conditions.

ELISA: Recombinant Human AFP Protein (rp169544)

Recombinant Human AFP Protein (rp169544) - ELISA
Immobilized Recombinant Human AFP Protein (rp169544) at 1.0 μg/mL can bind AFP Mouse mAb (Ab007318) with the EC₅₀ of 41.5 ng/mL.

References

1. Jintao Liang, Shuaikang Dong, Xiaohong Tan, Junyan Gong, Xuyang Chen, Zhide Zhou, Guiyin Li.  (2025)  A high-sensitivity label-free electrochemical aptasensor for point-of-care measurements of low-density lipoprotein in plasma based on aptamer and MXene-CMCS-Hemin nanocomposites.  BIOELECTROCHEMISTRY,      [PMID:40138878] [10.1016/j.bioelechem.2025.108972]

2. Guiyin Li, Bo Wang, Shengnan Li, Xinhao Li, Ruijie Yan, Xiaohong Tan, Jintao Liang, Zhide Zhou.  (2024)  Competitive electrochemical aptasensor for high sensitivity detection of liver cancer marker GP73 based on rGO-Fc-PANi nanocomposites.  BIOELECTROCHEMISTRY,      [PMID:38878458] [10.1016/j.bioelechem.2024.108767]

3. Liang Jintao, Yu Tingting, Tan Xiaohong, Jiang Lizhi, He Wei, Zhan Yulian, Huang Yong, Zhou Zhide, Li Guiyin.  (2024)  High-Precision Fluorescence Platform for Point-of-Care Measurements of Low-Density Lipoprotein Based on Nitrogen, Sulfur-Doped Graphene Quantum Dots and Ferric Tetroxide @ Reduced Graphene Oxide.  BioNanoScience,  15  (1): (1-14).  [PMID:] [10.1007/s12668-024-01618-3]

4. Xiao-Jian Li, Xinli Lu, Yunxia Wang, Zhanquan Zhang, Yongfa Zhang.  (2025)  Signal–off electrochemiluminescence immunosensor based on AgS quantum dots quenching luminol modified Ag/Cu2O/Ti3C2 nanocomposites for h–FABP detection.  NEW JOURNAL OF CHEMISTRY,      [PMID:] [10.1039/D5NJ02159F]