GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥90%(SDS-PAGE)
Expression System
HEK293
Accession #
P21815
Protein Tag
C-10His
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 of this effect is 0.06-0.6 μg/mL.
Animal Free,Carrier Free,Bioactive,ActiBioPure™,High Performance,His-Tag,≥90%(SDS-PAGE) ActiBioPure™,Animal Free,Bioactive,Carrier Free,High Performance,His Tag for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
Specifications
Product Name
Recombinant Human IBSP/Sialoprotein II Protein
Synonyms
Bone sialoprotein II | BSP II | Cell-binding sialoprotein | Integrin-binding sialoprotein | Bone sialoprotein 2 | BNSP | Bone sialoprotein | BSP 2 | BSP II | BSP | BSP2 | BSPII | BSP-II | Cell binding sialoprotein | IBSP | Integrin binding sialoprotein |
Grade
ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance, His Tag
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, His-Tag, ≥90%(SDS-PAGE)
Bioactivity
Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells. The ED50 of this effect is 0.06-0.6 μg/mL.
Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Human IBSP/Sialoprotein II Protein (rp143342) - SDS-PAGE 2 μg/lane of Recombinant Human IBSP/Sialoprotein II Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 70 - 110 kDa. The protein has a calculated MW of 35 kDa. The protein migrates as 70 - 110 kDa under reducing (R) and non-reducing (N) conditions due to glycosylation.
Recombinant Human IBSP/Sialoprotein II Protein (rp143342) - SDS-PAGE 2 μg/lane of Recombinant Human IBSP/Sialoprotein II Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 70 - 105 kDa. IBSP/Sialoprotein II is a highly glycosylated bone matrix protein, and its glycosylation modifications play a critical role in its biological functions and the mineralization processes of bone tissue. Structural characterization indicated that the known IBSP/Sialoprotein II are glycoproteins ranging in their apparent molecular weights between 70 - 105 kDa.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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