Recombinant Human IGF-I/IGF-1 Protein

Cat. No.: rp228953

GRADE & PURITY ≥95%(SDS-PAGE), See COA

Accession #

P05019

Protein Tag

No tag

Expression system

E. coli

Endotoxin Concentration

<1.0 EU/μg

Bioactivity

Measured in a cell proliferation assay using MCF-7 human breast cancer cell line. The ED50 for this effect is typically 34.3 ng/mL.

Specifications

Product Name

Recombinant Human IGF-I/IGF-1 Protein

Synonyms

IBP1 | IGF1 | IGF-1 | IGF1A | IGFI | IGF-I | IGF-IA | IGF-IB | insulin-like growth factor 1 (somatomedin C) | insulin-like growth factor 1 | insulin-like growth factor I | insulin-like growth factor IA | insulin-like growth factor IB | Mechano growth fact

Specifications & Purity

≥95%(SDS-PAGE), See COA

Biochemical and Physiological Mechanisms

Insulin-like growth factor 1 (IGF-1 or IGF-I), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate Cterminal prop

Bioactivity

Measured in a cell proliferation assay using MCF-7 human breast cancer cell line. The ED50 for this effect is typically 34.3 ng/mL.

Endotoxin Concentration

<1.0 EU/μg

Amino Acids

49-118 aa

Sequence

GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA

Accession #

P05019

Predicted molecular weight

7.7 kDa

SDS-PAGE

7.6 kDa, under reducing conditions; 7.6 & 15.7, under non-reducing conditions.

Molecule Type

Protein

Storage and Shipping

Concentration

See COA

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ for 12 months. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Protein Bioactivity: Recombinant Human IGF-I/IGF-1 Protein (rp228953)

Recombinant Human IGF-I/IGF-1 Protein (rp228953) - Protein Bioactivity

 Measured in a cell proliferation assay using MCF-7 human breast cancer cell line. The ED₅₀ for this effect is typically 9.08 ng/mL.

SDS-PAGE: Recombinant Human IGF-I/IGF-1 Protein (rp228953)

Recombinant Human IGF-I/IGF-1 Protein (rp228953) - SDS-PAGE 

3 μg/lane of Recombinant Human IGF-I/IGF-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 7.6 kDa under reducing conditions and 7.6 & 15.7 kDa under non-reducing conditions. IGF-I/IGF-1 can form a stable dimer on its own, which is mediated by a disulfide bond connecting the C-terminal helices of the α-subunits (referred to as α-CT for short) on the two monomers (PMID: 31594955).