Rhamnose Solution (20%, Sterile-Filtered) - Sterile-filtered, BioReagent, for Microscopy, 20% (w/v) in water , CAS No.10030-85-0

CAS: 10030-85-0 Cat. No.: R1524587 Molecular Weight: 182.17 Beilstein Registry Number: 3912643 EC Number: 600-058-2 PubChem CID: 20849066

GRADE & PURITY Sterile-filtered, BioReagent, for Microscopy, 20% (w/v) in water

Storage

Room temperature

Shipped In

Normal

Application

Microbial culture

Overview

L-(+)-Rhamnose can serve as a carbon source for microorganisms. Additionally, it acts as an inducer for rhamnose-inducible promoters, which is widely applied in prokaryotic expression systems such as the Lemo21(DE3) strain. This product is sterilized via filtration through a 0.22 μm membrane and can be directly added to culture media.

Product Composition:

Product Name	Product Component	Molecular Formula/CAS No./ Molecular Weight	Formula per Liter	Sterilization Method
Rhamnose Solution (20%, Sterile-Filtered)	L-(+)-Rhamnose	C₆H₁₂O₅/10030-85-0/182.17	200g	0.22um Filtration

Operation Protocol:

1. Determination of optimal rhamnose concentration for Lemo21(DE3) strain:

For proteins that are difficult to express, a concentration gradient test of rhamnose is required. Add rhamnose at different concentrations (0, 100, 250, 500, 750, 1000, 2000 µM) when inoculating the large-scale shake-flask culture. This step is optional; rhamnose may be omitted for readily expressible proteins.

Precautions

1. Unopened rhamnose solution can be stored at room temperature for 1 year. For long-term storage, preservation at -20 °C is recommended. Discard the product immediately if contamination is observed after opening.

2. Rhamnose solution cannot be sterilized by autoclaving/high-temperature sterilization.

Specifications

Specifications & Purity

Sterile-filtered, BioReagent, for Microscopy, 20% (w/v) in water

Stability And Storage

Store at room temperature long term (12 months).

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent, for Microscopy, Sterile-filtered

Names and Identifiers

• Isomeric SMILES: C[C@@H]([C@@H]([C@H]([C@H](C=O)O)O)O)O.O
• WGK Germany: 3
• Alternate CAS: 3615-41-6;6155-35-7
• PubChem CID: 20849066
• Molecular Weight: 182.17
• Beilstein: 3912643
• Reaxy-Rn: 4660356

Chemical and Physical Properties

• Melt Point(°C): 82-92°C

References

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