Ribonuclease H (RNase H)

Cat. No.: R754998

GRADE & PURITY from Escherichia coli H 560 pol A1

Accession #

P0A7Y0

Overview

Nonspecific endoribonuclease that specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides.

Source: E. coli H560 pol A1
Storage Buffer: 25 mM Tris-HCl, 50 mM KCl, 1 mM dithiothreitol, 0.1 mM EDTA, 50% glycerol (v/v), pH 8.0 (+4°C)
Volume Activity: 1 x 103 U/ml assayed according to Hillenbrand & Staudenbauer.

Ribonuclease H (RNase H) is a nonspecific endoribonuclease, localized to the nucleus and cytoplasm. It is ubiquitously found and widely present among many organisms including viruses and human.

Application:

Ribonuclease H (RNase H) has been used for:

• In vivo RNA-primed initiation of DNA synthesis
• Elimination of mRNA during second-strand cDNA synthesis
• Site-specific cleavage of RNA
• Detection of RNA:DNA regions in double-stranded DNA of natural origin
• Removal of poly (A) sequences of mRNA if oligo (dT) is present
• RNA extraction and quantitative reverse transcriptase polymerase chain reaction (RT-PCR)

Specifications

Product Name

Ribonuclease H (RNase H)

Specifications & Purity

from Escherichia coli H 560 pol A1

Biochemical and Physiological Mechanisms

Ribonuclease H (RNase H) specifically cleaves RNA in RNA:DNA hybrids. A minimum of four continuous base pairs (RNA:DNA) is required for activity. RNase H cleaves RNA to release 5′-oligoribonucleotides. RNase H is associated with nucleic acid immunity. Usi

Accession #

P0A7Y0

Molecule Type

Enzyme

Storage and Shipping

Concentration

from Escherichia coli H 560 pol A1

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -15–-25 °C. (unopened)