RPMI 1640 Medium (without Glucose) - BioReagent

Cat. No.: R1525852
AVAILABLE TO ORDER
GRADE & PURITY BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
Storage
Store at 2-8°C
Shipped In
Wet ice
Size
Status
Price
Qty
250ml
R1525852-250ml
8-12 wks(?) Production requires sourcing of materials. We appreciate your patience and understanding.
$19.90
500ml
R1525852-500ml
8-12 wks(?) Production requires sourcing of materials. We appreciate your patience and understanding.
$21.90
Enter a quantity for the sizes you want to add.
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Why this grade

BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

RPMI 1640 was developed by Moore et al. in 1967 at the Roswell Park Memorial Institute (RPMI) in Buffalo, New York, USA. RPMI is a series of cell culture media developed by this institute, and 1640 is the medium code. RPMI 1640 is a modified formulation of McCoy's 5A medium, utilizing a bicarbonate buffering system. Unlike most mammalian cell culture media, it is typically formulated at pH 8.0. Originally designed specifically for lymphocyte culture, RPMI 1640 is now widely used for the cultivation of various normal and cancer cells, particularly suspension cells, and has become one of the most commonly employed media. RPMI 1640 medium without glucose allows for flexible adjustment of glucose concentration according to experimental needs, which is convenient and rapid. This product contains multiple components required for cell culture, including amino acids, vitamins, inorganic salts, etc.; however, it contains no proteins, lipids, or any growth factors. Therefore, this product must be used with serum or serum‑free supplements. This product contains L‑glutamine (300 mg/L), NaHCO₃ (2000 mg/L), and phenol red (5 mg/L). It does not contain D‑glucose, sodium pyruvate, or HEPES. Precautions ① This product has been sterilized by filtration. Aseptic techniques must be observed during handling to prevent contamination. ② To maintain optimal performance, avoid freeze‑thaw cycles. ③ This product is intended for research or further study purposes only; it is not for diagnostic or therapeutic use.

Specifications

Specifications & Purity
BioReagent
Storage
Store at 2-8°C
Shipped In
Wet ice
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
BioReagent

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
Reviews

Customer Reviews

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