S Tag Peptide

Cat. No.: S750242

GRADE & PURITY ≥98%(HPLC), 5mg/ml

Overview

The S Tag Peptide is commonly used for competitive elution of S tag fusion proteins bound to S Tag antibodies. It can be used to elute S tag fusion proteins from Anti-S Tag Magnetic Beads or conventional S Tag antibodies during immunoprecipitation.This product competitively elutes the S Tag fusion protein, and the eluted sample does not contain the light and heavy chains of S Tag antibodies, thus effectively eliminating the interference of antibodies with subsequent Western blot analysis.The main parameters of S Tag Peptide are as follows

Precautions：

The solution of this product should be aliquoted and stored at -20℃ or lower temperatures to avoid repeated freeze-thaw cycles.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. For 25mg provided in powder form, centrifuge at 8,000-12,000×g for 10-30 seconds before opening to collect the powder at the bottom of the tube. Dissolve it in TBS by gently pipetting or shaking. Do not vortex vigorously to avoid denaturation and inactivation of the peptide.2. P9816-1mg and P9816-5mg can be directly used for preparing elution buffer. 3. Competitive elution with the S Tag Peptide: This elution method is non-denaturing, with high elution efficiency, and the eluted sample does not contain the light and heavy chains of S Tag antibodies.a. Preparation of S tag elution buffer: Based on the required amount of elution buffer, dilute an appropriate amount of S Tag Peptide stock solution with TBS to a final concentration of 150μg/ml.b. Add 100μl of S tag elution buffer (150μg/ml) to each immunoprecipitated sample, and incubate on ice or at 4℃ for 30 minutes to 2 hours with shaking. In order to improve the elution efficiency, the incubation time can be extended or the elution can be repeated. The volume of the S tag elution buffer is generally 5 times that of the bead or gel suspension.c. Centrifuge at 6000×g for 30 seconds at 4℃, and carefully transfer the supernatant to a new tube. The supernatant contains the S tag fusion protein and its protein complexes. Be careful not to touch the resin when taking the supernatnant. 4. Store the eluted S tag fusion protein at 4℃ for immediate use, or at -20℃ for long-term storage.

Specifications

Product Name

S Tag Peptide

Specifications & Purity

≥98%(HPLC), 5mg/ml

Molecule Type

Peptide

Storage and Shipping

Concentration

5mg/ml

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Stored at 4℃ for up to 1 year.