UDP-sugar pyrophosphorylase (AtUSP) , CAS No.223918-15-8

CAS: 223918-15-8 Cat. No.: U1520614

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), Protein concentration: See COA; ≥500 U/mg protein

Accession #

Q9C5I1

Protein Tag

N-His

Expression system

E. coli

Bioactivity

≥50 U/mg protein

Overview

  UDP-sugar pyrophosphorylase (AtUSP, E.C. 2.7.7.64), derived from Arabidopsis thaliana.In combination with inorganic pyrophosphatase and Galactokinase (Cat. No. G1434912) , this enzyme is used to synthesize UDP-galactose, UDP-glucose, UDP-mannose and their derivatives, which serve as glycosyl donors for glycosyltransferases. It acts as a tool enzyme for the synthesis of sugar nucleotides such as UDP-gal, UDP-Glc and UDP-Man.This product is recombinantly expressed in Escherichia coli, featuring high purity and good compatibility, suitable for one-pot multi-enzyme systems. 

Reaction: 

Protocol for Use

1. Add substrates to the reaction mixture, supplemented with 5 mM MgCl₂. An appropriate buffer (e.g., Tris-HCl) may be added to adjust the pH to 7.5, followed by the addition of an appropriate amount of AtUSP. Optionally, PmPPA may be added to promote the reaction.
2. Mix thoroughly and incubate the sample at 37°C for 1–2 hours (adjust based on substrate conditions).

Note: For scaled-up reactions, adjust the incubation time or enzyme amount based on actual conditions. Gentle shaking may be used for mixing.

Note: Most common biological buffers are applicable, such as Tris or PBS. Buffers outside this pH range (e.g., acetate buffer) may also be applicable, but the incubation time or enzyme amount needs to be optimized according to actual conditions.

Precautions

For your safety and health, please wear lab coats and disposable gloves when handling. This product is for research use only!

Specifications

Product Name

UDP-sugar pyrophosphorylase (AtUSP) , CAS No.223918-15-8

Synonyms

UTP-monosaccharide-1-phosphate uridylyltransferase | UDP-sugar pyrophosphorylase | USP | ATUSP | F6N7.4 | F6N7_4

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), Protein concentration: See COA; ≥500 U/mg protein

Biochemical and Physiological Mechanisms

Required for the synthesis of the intine, the pectocellulosic inner wall of developing pollen. May function as the terminal enzyme of the myo-inositol oxidation (MIO) pathway. May also play a role in the salvage pathway for synthesis of nucleotide sugars.

Bioactivity

≥50 U/mg protein

Accession #

Q9C5I1

Predicted molecular weight

67.9 kDa

CAS

223918-15-8

Enzyme Commission Number

EC 2.7.7.64

Molecule Type

Enzyme

Storage and Shipping

Concentration

Protein concentration: See COA; ≥500 U/mg protein

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

One unit is defined as the amount of enzyme that catalyzes the production of 1 μmol of UDP-Gal from Gal-1-P and UTP per minute at 37°C.