BioReagent,for IP,50% v/v BioReagent,for IP for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Aladdin's UltraBio™ Anti-MBP Agarose is produced by high-quality MBP antibodies to 4% agarose gel. These agarose can specifically bind MBP fusion proteins in lysates, serum, ascites, or other samples from animals, plants, or microorganisms. This product is mainly used for immunoprecipitation (IP), co-immunoprecipitation (Co-IP), or purification of MBP-tagged proteins or their protein complexes. Maltose binding protein (MBP) encoded by the malE gene is a component of the E. coli maltose/maltodextrin system and regulates the uptake and catabolism of maltodextrins, with a molecular weight of approximately 42kDa [1]. The MBP tag can be fused to the N- or C-terminus of a protein, with the N-terminus being the most common fusion site. The MBP tag has several advantages: 1. Increased solubility of heterologous proteins.2. Wide applicability in different hosts/systems.3. Facilitation of proper protein folding. 4. High specificity allowing for mild and convenient purification. 5. Versatile tool for downstream analysis: Following fusion, the expression, localization, and function of the target protein can be analyzed, or the protein can be purified, immunoprecipitated (IP), or co-immunoprecipitated (Co-IP) using MBP-specific antibodies or Anti-MBP Agarose gel.
Aladdin UltraBio™ Anti-MBP Agarose is stored in PBS containing 0.02% ProClin 300, with a settled gel to storage solution ratio of 1:1(This product is a 50% gel suspension, containing 0.5 ml of settled agarose per milliliter. Our product specifications are based on total volume. Product information is detailed in the table below:
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Instruction for use
1. Sample Preparation
1.1 Ensure the sample solution has appropriate ionic strength and pH before loading. Dilute the sample or cell culture supernatant with equilibration buffer, or dialyze the sample against equilibration buffer.
1.2 Clarify the sample by centrifugation or filtration through a 0.22 μm or 0.45 μm membrane to reduce impurities, improve purification efficiency, and prevent column clogging.
1.3 Preparation of protein samplesa.Lysis cells or tissues with appropriate lysis buffer, such as ’s Cell Lysis Buffer for Western and IP . Under certain circumstances, ’s RIPA Strong Lysis Buffer , RIPA Medium Lysis Buffer , or RIPA Weak Lysis Buffer can be used. Other lysis buffers with a pH6-8 can also be used
2. Buffer Preparation
It is recommended to filter water and buffers through a 0.22 μm or 0.45 μm membrane before use.
Equilibration Buffer: 0.01M PBS，pH7.4；
Wash Buffer: 0.01M PBS，0.05% Tween-20，pH7.4
3. Immunoprecipitation (IP) Procedure
3.1 Resin Preparation: Add 40 µL of Anti-MBP Agarose suspension (20 µL settled agarose) to a 1.5 mL tube. Centrifuge at 5,000 × g for 1 min. Discard the supernatant.
3.2 Add 0.5 mL of Equilibration Buffer to resuspend the agarose (Place the agarose in the same buffer system as the target protein to protect the protein.). Centrifuge at 5,000 × g for 1 min. Discard the supernatant. Repeat this wash step once.
3.3 Add 300 µL of sample lysate to the prepared agarose from step 3.2. Mix and incubate on a tube rotator at room temperature for 3 hours. Centrifuge at 5,000 × g for 1 min. Discard the supernatant.
3.4 Washing: Add 0.5 mL of Wash Buffer, resuspend the agarose, and mix gently. Centrifuge at 5,000 × g for 1 min. Discard the supernatant. Repeat this wash step three more times.
3.5 Denaturing Elution (SDS-PAGE): Add 100 µL of 1× Loading Buffer to the agarose. Heat at 95°C for 5 min. Centrifuge at 5,000 × g for 1 min, and load the supernatant directly onto an SDS-PAGE gel for analysis.
Note: The Standard loading buffer used in laboratories contains β-mercaptoethanol and DTT, which can break the disulfide bonds between the antibody heavy and light chains on the agarose. This method denatures the antibody, rendering the resin unusable for reuse.
Matters needing attention
1. This product must be fully resuspended by inverting the tube several times prior to use.
2. For immunoprecipitation assays, it is recommended to include both positive and negative controls.
3. Protein samples should be purified as soon as possible after collection and should always be placed at 4℃ or on ice to minimize protein degradation or denaturation.
4. The non-specific binding capability of this product can be affected by high concentrations of DTT, mercaptoethanol, and guanidine hydrochloride, but it is applicable in Cell lysis buffer for Western and IP , RIPA Lysis Buffer , and NP-40 Lysis Buffer .
5. Protein degradation can also be inhibited by adding appropriate protease inhibitors, such as Protease Inhibitor Cocktail for General Use , Protease and Phosphatase Inhibitor Cocktail for General Use (MS-safe, 50X) , Protease Inhibitor Cocktail for Mammalian Cell and Tissue Extracts , Protease and Phosphatase Inhibitor Cocktail for Mammalian Cell and Tissue Extracts , etc.
6. This product is for R&D only. Not for drug, household, or other uses.
7. For your safety and health, please wear a lab coat and disposable gloves during the operation.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
 ZJ26F0434639 | Certificate of Analysis | Apr 24, 2026 | U1508192 |
| ZJ26F0434638 | Certificate of Analysis | Apr 24, 2026 | U1508192 |
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