GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
α-Ketoglutarate (α-KG) is an important intermediate metabolite in the tricarboxylic acid (TCA) cycle, positioned after isocitrate and before succinyl-CoA. It links carbon and nitrogen metabolism, directly participating in oxidative energy production and the biosynthesis of various substances, thus playing a vital role in maintaining normal physiological functions. This kit provides a rapid and sensitive method for the determination of α-KG: Under the catalysis of alanine aminotransferase (ALT), α-KG is converted to glutamate. The glutamate is then further reacted by glutamate dehydrogenase (GDH) to generate NADH. The generated NADH reacts with a chromogenic reagent to produce a yellow product with a characteristic absorbance peak at 450 nm. By measuring the absorbance at this wavelength, the α-KG content in the sample can be precisely calculated.
Storage and Shipping
Storage
Store at 2-8°C
Shipped In
Wet ice
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
