ADP-specific Glucokinase, CAS No.173585-07-4

CAS: 173585-07-4 Cat. No.: A1439124 EC Number: 838-695-5

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), Protein concentration: See COA; ≥500 U/mg protein

Accession #

Q9V2Z6

Protein Tag

N-His, C-His

Expression system

E. coli

Bioactivity

≥500 U/mg protein

Overview

ADP-specific Glucokinase is an ADP-dependent glucokinase (EC 2.7.1.147), with its gene derived from Pyrococcus furiosus. It exists as a dimer, with a monomer molecular weight of 47 kD. It uses ADP as the phosphate donor to catalyze the conversion of glucose to glucose-6-phosphate. This product is recombinantly expressed in E. coli, can tolerate temperatures up to 90°C, and has an optimal temperature of 50°C.

Reaction formula

Glucose + ADP →Glucose-6-phosphate + AMP  

Product Properties

Source: Expressed in E. coli

Label: N-His & C-His

Purity: ≥ 90% (analyzed by SDS-PAGE)

Molecular weight: 100 kDa

Buffer: 20 mM Tris-HCl, 500 mM NaCl, 10% Glycerol, pH 7.5.

Activity: ≥500 U/mg protein

Unit definition: One unit of enzyme activity is defined as the amount of enzyme required to consume 1.0 μmol of ADP per minute under conditions of 50°C and pH 7.5.

Usage Instructions

1. Add the substrate and 10 mM MgCl₂ to the reaction system; adjust the pH to 7.5 with appropriate buffer salts, then add a suitable amount of ADP-specific Glucokinase (as needed for the reaction);

2. After mixing, incubate the sample at 50°C for 4 hours or overnight.

【Note】 When scaling up the reaction system, adjust the incubation time or enzyme dosage according to actual conditions; low-speed shaking can be used for mixing.

【Note】 Most common biological buffers (e.g., Tris or PBS) are applicable. Buffers outside this pH range (e.g., acetate buffer) may be usable, but the incubation time or enzyme dosage needs to be optimized based on actual conditions.

Precautions

For your safety and health, wear a lab coat and disposable gloves during operation. This product is for research use only!

Specifications

Product Name

ADP-specific Glucokinase, CAS No.173585-07-4

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), Protein concentration: See COA; ≥500 U/mg protein

Biochemical and Physiological Mechanisms

Catalyzes the ADP-dependent phosphorylation of D-glucose to D-glucose 6-phosphate and glucosamine to glucosamine 6-phosphate. Can also use CDP as the phosphoryl group donor and D-1, 5-anhydroglucitol as the phosphoryl group acceptor.

Bioactivity

≥500 U/mg protein

Accession #

Q9V2Z6

Predicted molecular weight

100 kDa

CAS

173585-07-4

Molecule Type

Enzyme

Storage and Shipping

Concentration

Protein concentration: See COA; ≥500 U/mg protein

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

The amount of enzyme required to consume 1.0 μmole of ADP per minute at 50 ºC and pH 7.5.