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beta Actin Mouse mAb (AF405) - Primary antibody, specific to beta actin

Cat. No.: Ab219181
COA
beta Actin Mouse mAb (AF405) - by Aladdin Scientific
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
Flow Cytometry
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG1
Clone Number
FG01/2D10
Conjugation
AF405
★
Size
Status
Price
Qty
10μl
Ab219181-10μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$53.90
50μl
Ab219181-50μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$145.90
100μl
Ab219181-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$227.90
1ml
Ab219181-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$1,499.90
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SDS
Safety data
COA
Certificate
DS
Datasheet
SP
Spec sheet
Overview Specifications Storage and Shipping Images Documentation Advanced Data Associated Targets(Human) Certificates（CoA,COO,BSE/TSE and Analysis Chart) Documents & Articles Solution Calculators Reviews
🧪

Why this grade

ExactAb™, Validated, 0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human beta actin Antibody, Monoclonal (FG01/2D10), could be used for Flow Cytometry.

Specifications

Product Name
beta Actin Mouse mAb (AF405) - Primary antibody, specific to beta actin
Synonyms
A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | β actin antibody |
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Host species
Mouse
Specificity
beta actin
Immunogen
Synthetic peptide in beta Actin
Positive Control
Flow Cytometry: Jurkat cells
Conjugation
AF405
Excitation (nm)
Violet Laser(405nm)
Emission(nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Images

beta Actin Mouse mAb (AF405) (Ab219181) - Flow Cytometry
Flow cytometry analysis of beta Actin expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with beta Actin Mouse mAb (AF405) (Ab219181) (Red) at 1/300 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF405) (Ab190076).

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
ACTB Tbio Actin, cytoplasmic 1 (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ25F0724300Certificate of AnalysisMay 20, 2026 Ab219181
ZJ25F0724301Certificate of AnalysisMay 20, 2026 Ab219181
ZJ25F0724302Certificate of AnalysisMay 20, 2026 Ab219181
Documents & Articles
Main Methods and Experimental Selection for Microfilament Staining
Categories: Technical articles
Tags:
Immunofluorescence cell migration microfilament staining F-actin actin phalloidin cytoskeleton stress fibers super-resolution imaging
Solution Calculators
Reviews

Customer Reviews

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