GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Native?Native grade — protein/biomolecule in its natural (non-recombinant, non-denatured) form. Use when native structure and activity are required.
BioReagent, Native BioReagent,Native for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
The carbohydrate binding site recognizes (β-1,4) linked N-acetylglucosamine oligomers, preferring chitobiose or chitotriose over a single N-acetylglucosamine residue. This lectin binds well in the acidic pH range but its affinity decreases above pH 8.0. DSL also binds well to N-acetyllactosamine and oligomers containing repeating N-acetyllactosamine sequences. A branched pentasaccharide including two N-acetyllactosamine disaccharides linked to mannose (β-1,6) and (β-1,2) was reported to be the most potent inhibitor of agglutination.
DSL contains two chains of 40 kDa and 46 kDa joined by disulfide bonds. This lectin is free of a reported 32 kDa contaminant protein.
Inhibiting/Eluting Sugar: Chitin Hydrolysate
Specifications
Molecular Weight
86 kDa
Extinction Coefficient
0.6
Inhibiting or Eluting Sugar
Chitin Hydrolysate or Desialylated Fetuin
Storage Instructions
2-8 °C; for long term storage, aliquots may be stored frozen or preserved with 0.08% sodium azide in the recommended buffer and stored at 2-8 °C.
Sugar Specificity
Branching structures with polyLacNAc
Usage Summary
Though many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl₂ is recommended. For preserving solutions stored at 4 °C, 0.08% sodium azide can be used. Aggregation may occur with time if stored at concentrations greater than 2 mg/ml.
Applications
Glycobiology, Mitogenic Stimulation
Conjugate
Unconjugated
Specifications
Product Name
Datura Stramonium Lectin (DSL)
Synonyms
Lectin from Datura stramonium
Grade
BioReagent, Native
Specifications & Purity
BioReagent, Native
Conjugation
Unconjugated
Predicted molecular weight
86 kDa
Molecule Type
Protein
Storage and Shipping
Reconstitution
Reconstitute in 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl₂. For preserving solutions stored at 4 °C, 0.08% sodium azide can be used. Aggregation may occur with time if stored at concentrations greater than 2 mg/ml.
Storage
Store at 2-8°C
Shipped In
Wet ice
Stability And Storage
Store at 2-8℃ long term. Upon reconstitution, it is recommended to aliquot.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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