EHA101 Agrobacterium tumefaciens Glycerol Stock (for Transgenic Plant Construction) - BioReagent,bacteriological grade

Cat. No.: E745547

GRADE & PURITY BioReagent, bacteriological grade

Synonyms

Agrobacterium EHA101 | EHA101 agrobacterium tumefaciens glycerol stock for construction of transgenic plant | EHA101 Agrobacterium tumefaciens

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Application

586

Overview

The EHA101 strain is derived from the C58 background, with a screening marker—the rifampicin resistance gene Rif—present in its nuclear genome. For facilitating transformation operations, this strain carries a nopaline-type Ti plasmid pEHA101 (pTiBo542ΔT-DNA) that lacks self-transport functionality. This plasmid contains vir genes, which are essential components for the insertion of T-DNA into the plant genome. Although the T-DNA transfer function of the pEHA101 (pTiBo542ΔT-DNA) plasmid itself is disrupted, it can assist in the smooth transfer of T-DNA from the introduced binary vector. The pEHA101 (pTiBo542ΔT-DNA) type Ti plasmid contains a screening marker: Kan, which endows the EHA101 strain with kanamycin resistance. It is suitable for transgenic operations in plants such as maize, rice, and tobacco.

Product Usage: Agrobacterium tumefaciens strain

Prokaryotic Resistance: Kan+, Rif+ (Rif+ resistance causes the strain to grow slowly)

Genotype: C58 (rif^R) Ti pEHA101 (pTiBo542 D T-DNA) (kan^R) Nopaline

Culture Medium: LB Medium

Culture Conditions: 28–30°C, aerobic

Product Specification: 200 μL Glycerol Stock

Strain Amplification: Take 100-200 μl of the glycerol stock, inoculate it into 3-5 ml of fresh LB medium (with Kan+ resistance), and incubate with shaking overnight at 28-30°C. Alternatively, you can first streak the glycerol stock on an agar plate, then pick a single colony, and incubate with shaking overnight.

Specifications

Synonyms

Agrobacterium EHA101 | EHA101 agrobacterium tumefaciens glycerol stock for construction of transgenic plant | EHA101 Agrobacterium tumefaciens

Specifications & Purity

BioReagent, bacteriological grade

Stability And Storage

Store at -80℃ long term (12 months). Avoid freeze/thaw cycle.

Storage

Store at -80°C, Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

bacteriological grade, BioReagent