Heparinase I and III blend

Cat. No.: H766347

GRADE & PURITY EnzymoPure™, ≥200(U/mg)，from Flavobacterium heparinum

Overview

Hepases, derived from microorganisms, are polysaccharide lyases that cleave the a-1,4 glycosidic bond between N-acetylglucosamine (GlcNAc) and hexuronic acid (GlcUA/IdoUA) through a typical b-elimination mechanism. They generate an unsaturated double bond with specific absorption at 232 nm at the C4 and C5 positions of hexuronic acid, which facilitates the analysis and detection of enzymatic hydrolysis products. HEPases have been identified in Hep/HS degrading bacteria, such as Flavobacterium heparinum, Bacteroides thetaiotaomicron，Bacteroides stercoris，Sphingomonas, Bacillus , Pseudomonas aeruginosa[ Wait. HEPases can be divided into three categories based on substrate selectivity: HEPase I selectively degrades the high sulfation zone in Hep and HS; HEPase III selectively degrades the low sulfur acidification zone in HS and Hep; HEPase II can degrade both Hep and HS simultaneously. The above three types of HEPases belong to endonucleases, and recently a heparin exonuclease family (exoHEPases) has been discovered for the first time. HEPases, as an important tool enzyme, are widely used in the structural and functional research of Hep/HS, the production of low molecular weight heparin, quality testing and consistency evaluation of heparin drugs, etc. We can provide customers with various known types of HEPase enzyme preparations according to their needs, meeting their various requirements from analysis and detection to large-scale production.

Application

Heparinase I and III Blend from Flavobacterium heparinum has been used in:the digestion of heparan sulfate from ovine vitreous；human embryonic kidney cells；glycosaminoglycans from arterial tissues；P0 retinae digestion.

Specifications

Product Name

Heparinase I and III blend

Grade

EnzymoPure™

Specifications & Purity

EnzymoPure™, ≥200(U/mg)，from Flavobacterium heparinum

Biochemical and Physiological Mechanisms

Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate. Heparinase I and III plays vital role in various biological processes: modulate cell-growth factor interactions, cell-lipoprotein interactions, neovascularizati

Enzyme Commission Number

4.2.2.7 (BRENDA, IUBMB)

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥200(U/mg)，from Flavobacterium heparinum

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Unit definition

One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using Heparin, Sodium as substrate for heparinase I. One unit will form 0.1 micromole of unsaturated uronic acid per hour at 7.5 at 25 degrees C using bovine kidne