L-Leucine-7-amido-4-methylcoumarin hydrochloride - ≥99%(HPLC) , CAS No.62480-44-8

CAS: 62480-44-8 Cat. No.: L131532 Molecular Weight: 324.80

GRADE & PURITY ≥99%(HPLC)

Synonyms

[(1S)-3-Methyl-1-[(4-Methyl-2-Oxo-Chromen-7-Yl)Carbamoyl]Butyl]Ammonium | (2S)-2-Amino-4-Methyl-N-(4-Methyl-2-Oxo-2H-1-Benzopyran-7-Yl)-Pentanamide Monohydrochloride | [(1S)-3-Methyl-1-[[(4-Methyl-2-Oxo-7-Chromenyl)Amino]-Oxomethyl]Butyl]Ammonium | [(1S)-

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

L-Leucine 7-amido-4-methylcoumarin hydrochloride is a fluorogenic substrate for LAP3 (leucine aminopeptidase). Upon enzyme activity, a bright blue fluorescent dye, 7-amino-4-methylcoumarin, is released.
Leu-AMC.HCl, a fluorogenic substrate for leucine aminopeptidase

Specifications

Synonyms

[(1S)-3-Methyl-1-[(4-Methyl-2-Oxo-Chromen-7-Yl)Carbamoyl]Butyl]Ammonium | (2S)-2-Amino-4-Methyl-N-(4-Methyl-2-Oxo-2H-1-Benzopyran-7-Yl)-Pentanamide Monohydrochloride | [(1S)-3-Methyl-1-[[(4-Methyl-2-Oxo-7-Chromenyl)Amino]-Oxomethyl]Butyl]Ammonium | [(1S)-

Specifications & Purity

≥99%(HPLC)

Biochemical and Physiological Mechanisms

Fluorogenic substrate for leucine aminopeptidase.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Purity

≥99%(HPLC)

Product Properties

• Excitation and Emision Ranges: λex 325 nm,λem 397 nm in 0.1 M Tris pH 8.0;λex 380 nm,λem 440 nm (reaction product);λex 327 nm,λem 349 nm in pH 8.0;λex 325 nm,λem 395 nm

Names and Identifiers

• Canonical Smiles: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CC(C)C)N.Cl
• IUPAC Name: (2S)-2-amino-4-methyl-N-(4-methyl-2-oxochromen-7-yl)pentanamide;hydrochloride
• InChIKey: VCRXITKKWBOQRZ-ZOWNYOTGSA-N
• INCHI: 1S/C16H20N2O3.ClH/c1-9(2)6-13(17)16(20)18-11-4-5-12-10(3)7-15(19)21-14(12)8-11;/h4-5,7-9,13H,6,17H2,1-3H3,(H,18,20);1H/t13-;/m0./s1
• Isomeric SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)[C@H](CC(C)C)N.Cl
• Molecular Weight: 324.80

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives
            • Level7 Leucine and derivatives

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives - Alpha amino acids and derivatives
• Direct Parent: Leucine and derivatives
• Alternative Parents: Alpha amino acid amides Coumarins and derivatives 1-benzopyrans N-arylamides Pyranones and derivatives Benzenoids Fatty amides Heteroaromatic compounds Secondary carboxylic acid amides Lactones Oxacyclic compounds Hydrocarbon derivatives Hydrochlorides Carbonyl compounds Monoalkylamines Organic oxides Organopnictogen compounds
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Leucine or derivatives - Alpha-amino acid amide - Coumarin - Benzopyran - 1-benzopyran - N-arylamide - Pyranone - Fatty amide - Pyran - Fatty acyl - Benzenoid - Heteroaromatic compound - Carboxamide group - Lactone - Secondary carboxylic acid amide - Oxacycle - Organoheterocyclic compound - Hydrocarbon derivative - Primary amine - Organooxygen compound - Organonitrogen compound - Primary aliphatic amine - Amine - Organic oxide - Organopnictogen compound - Organic nitrogen compound - Carbonyl group - Organic oxygen compound - Hydrochloride - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as leucine and derivatives. These are compounds containing leucine or a derivative thereof resulting from reaction of leucine at the amino group or the carboxy group, or from the replacement of any hydrogen of glycine by a heteroatom.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in methanol at 50mg/ml
• Sensitivity: light & moisture sensitive
• Melt Point(°C): 283℃
• Molecular Weight: 324.800 g/mol
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 4
• Exact Mass: 324.124 Da
• Monoisotopic Mass: 324.124 Da
• Topological Polar Surface Area: 81.400 Ų
• Heavy Atom Count: 22
• Formal Charge: 0
• Complexity: 445.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 1
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 2

XLogP3

References

1. Kan Chen, Rui Yang, Min Li, Yanan Chen, Xiurong Han, Jing Zhao, Keqiang Li, Xiulin Wang.  (2024)  The Hydrophobic Component of Terrestrial Dissolved Organic Nitrogen Promotes Shifting Diatom–Dinoflagellate Dominance in the Bohai Sea.  JOURNAL OF GEOPHYSICAL RESEARCH-OCEANS,  129  (10): (e2024JC021373).  [PMID:] [10.1029/2024JC021373]

2. Xinyu Li, Cui Zeng, Xiurong Han, Liqiang Fan, Jing Zhao, Yingzhe Wang, Xiulin Wang, Keqiang Li.  (2025)  The effect of the aging processes of Ulva prolifera-derived dissolved organic nitrogen associated with green tide on the diatoms-dinoflagellates succession in the Southern Yellow Sea, China.  WATER RESEARCH,      [PMID:40010127] [10.1016/j.watres.2025.123370]