Lumi-Light PLUS Western Blotting Substrate

Cat. No.: L755436

GRADE & PURITY pkg of 100 mL (1,000 cm2 membrane)

Overview

As the technology for quantifying chemiluminescence advances, the substrates for chemiluminescence must also advance. For instance, the light emission of conventional enhanced luminol substrates substantially decreases within 30 minutes. In contrast, the chemiluminescence signal of the Lumi-LightPLUS substrate persists for more than nine hours, and since exposures of only a few minutes are necessary, multiple exposures can be taken. Additionally, the ability to take extended exposures allows the detection of rare proteins, or higher dilutions of antigen or antibody to be used. The emission wavelength of the Lumi-LightPLUS substrate is 425 nm.

Comparison of Lumi-Light Substrate and Lumi-Light PLUS Substrate

Lumi-Light Substrate Lumi-Light^PLUS Substrate
Sensitivity 10-50 pg antigen 1-5 pg antigen
Pimary Antibody Dilution Dilute up to 10-fold more compared to colorimetric detection Dilute up to 100-fold more compared to colorimetric detection
Signal Stability >3 hours (enhance signal with a 30-minute incubation) > 9 hours (enhance signal with a 30-minute incubation)

Number of tests: The reagent is sufficient for 10 blots, each with a size of 10 cm x 10 cm.
Sensitivity: Depending on the affinity of the primary antibody, 1 - 5 pg of antigen can be detected in research studies.
Storage/stability: The Lumi-LightPLUS substrate components are stable at +2 to +8°C until the expiration date printed on the label. Components are shipped at +15 to +25°C.

Application:

Lumi-Light^PLUS Western Blotting Substrate has been used in western blotting.

Lumi-Light^PLUS Western Blotting Substrate is for the chemiluminescent detection of antigens blotted on membranes using horseradish peroxidase-conjugated secondary antibodies. It can be used for very sensitive detection of any antigen blotted on a PVDF Western Blotting Membrane or nitrocellulose membrane.

Lumi-Light^PLUS Western Blotting Substrate is suited for high-sensitivity western blotting, especially when quantification is required. The chemiluminescent signal persists for more than 9 hours, allowing multiple exposures to be taken.

Specifications

Product Name

Lumi-Light PLUS Western Blotting Substrate

Specifications & Purity

pkg of 100 mL (1, 000 cm2 membrane)

Molecule Type

Bioreagents/Buffer

Storage and Shipping

Concentration

pkg of 100 mL (1,000 cm2 membrane)

Storage

Store at 2-8°C

Shipped In

Wet ice