BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
This kit employs a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of Rat β-NGF concentration in samples. The Rat β-NGF capture antibody is pre-coated onto the microtiter plate. Upon addition of samples or standards, Rat β-NGF present therein binds to the immobilized capture antibody, while other unbound components are removed through washing. Subsequently, a biotin-conjugated detection antibody specific for Rat β-NGF is added. This antibody binds to the captured Rat β-NGF, forming a "sandwich" immunocomplex. Any excess, unbound material is again removed by washing. Next, an enzyme conjugate (typically Streptavidin-Horseradish Peroxidase, SA-HRP) is added. The biotin on the detection antibody and streptavidin on the enzyme conjugate exhibit high-affinity binding, thereby linking the HRP enzyme to the immunocomplex. Following another wash step to remove unbound conjugate, a colorimetric substrate (e.g., TMB) is added. If Rat β-NGF is present in the sample, the HRP linked to the complex catalyzes the oxidation of the colorless substrate, yielding a blue product. The reaction is then stopped by adding a stop solution (usually an acid), which changes the color from blue to yellow. The optical density (OD) of each well is measured at 450 nm using a microplate reader. The concentration ofRat β-NGF is directly proportional to the OD450 value. A standard curve is generated by assaying known concentrations ofRat β-NGF, and the concentration of Rat β-NGF in unknown samples is interpolated from this curve based on their OD values.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View BioReagent grade guide →
